尿嘧啶雙磷酸葡萄糖和鳥糞□呤雙磷酸甘露糖為細菌合成脂多醣、莢膜以及菌體周邊寡醣等致病相關構造中重要的前驅物。負責合成此些核苷酸糖的酵素，分別為尿嘧啶雙磷酸葡萄糖聚磷酸酵素和磷酸甘露糖異位轉化酶/鳥糞□呤雙磷酸甘露糖聚磷酸酵素。為了瞭解這些酵素的功能區域是否能夠互換，首先由綠膿桿菌 (Pseudomonas aeruginosa PAO1) 中選殖出UDPGP與可能具有雙功能性 (PMI/GMP) 之蛋白WbpW，然後利用domain shuffling技術，根據蛋白質鍵結區域性的不同特質，分別建構具有不同受質接受位的鑲嵌酵素。這些鑲嵌蛋白的功能測試分成兩個階段進行。首先，將新建構好的質體送入缺乏尿嘧啶基因的大腸桿菌菌株 FF4001中，檢測其在含有0.4%半乳糖的MacConkey agar上是否具有復原該突變株使用半乳糖的能力；接著，再以酵素動力學方式針對這些鑲嵌蛋白質進行不同受質專一性的檢測。目的為找出鑲嵌蛋白是否保有原生型蛋白受質鍵結區域，或是鑲嵌蛋白具有辨認新受質之能力。結果發現原生型蛋白與鑲嵌蛋白YW009、YW011均具有PMI功能，其對M-6-P之Km值分別為0.64 mM、7.9 mM和250 mM。而鑲嵌蛋白在PMI活性上有明顯減低，可知位於蛋白質C端區域參與PMI活性作用。因此，可利用藥物阻塞C端蛋白質的活性，以破壞多醣體的合成，減低細菌的致病力。

Baltimore, R. S. and M. Mitchell (1980). "Immunologic investigations of mucoid strains of Pseudomonas aeruginosa: comparison of susceptibility to opsonic antibody in mucoid and nonmucoid strains." J Infect Dis 141(2): 238-47.
Chang, H. Y., H. C. Huang, et al. (1999). "Characterization of a putative Pseudomonas UDPglucose pyrophosphorylase." Proc Natl Sci Counc Repub China B 23(2): 74-84.
Chang, H.Y., Deng, W.L., Lee, J.H., Fu, T.F. and Peng, H.L.(1996) " Vrulence and outer membrane properties of a Klebsiella pneumoniae galU mutant". Microb.Pathogen., 20: 255-261
Crater, D. L. and I. van de Rijn (1995). "Hyaluronic acid synthesis operon (has) expression in group A streptococci." J Biol Chem 270(31): 18452-8.
Davies, J. C. (2002). "Pseudomonas aeruginosa in cystic fibrosis: pathogenesis and persistence." Paediatr Respir Rev 3(2): 128-34.
Degeest, B. and L. de Vuyst (2000). "Correlation of activities of the enzymes alpha-phosphoglucomutase, UDP-galactose 4-epimerase, and UDP-glucose pyrophosphorylase with exopolysaccharide biosynthesis by Streptococcus thermophilus LY03." Appl Environ Microbiol 66(8): 3519-27.
Dever, T. E., M. J. Glynias, et al. (1987). "GTP-binding domain: three consensus sequence elements with distinct spacing." Proc Natl Acad Sci U S A 84(7): 1814-8.
Dillard, J. P., M. W. Vandersea, et al. (1995). "Characterization of the cassette containing genes for type 3 capsular polysaccharide biosynthesis in Streptococcus pneumoniae." J Exp Med 181(3): 973-83.
Evans, L. R. and A. Linker (1973). "Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa." J Bacteriol 116(2): 915-24.
Fukasawa, T., Jokura, and Kurahashi, K. (1962) "A new enzymic defect of galactose metabolism in Escherichia coli K-12 mutants ". Biochem. Biophys. Res. Commun., 7 121-125
Giaever, H. M., O. B. Styrvold, et al. (1988). "Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli." J Bacteriol 170(6): 2841-9.
Govan, J. R. and V. Deretic (1996). "Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia." Microbiol Rev 60(3): 539-74.
Govan, J. R. and J. A. Fyfe (1978). "Mucoid Pseudomonas aeruginosa and cystic fibrosis: resistance of the mucoid from to carbenicillin, flucloxacillin and tobramycin and the isolation of mucoid variants in vitro." J Antimicrob Chemother 4(3): 233-40.
Gustafson, G. L. and J. E. Gander (1972). "Uridine diphosphate glucose pyrophosphorylase from Sorghum vulgare. Purification and kinetic properties." J Biol Chem 247(5): 1387-97.
Hoiby, N., L. Jacobsen, et al. (1974). "Pseudomonas aeruginosa infection in cystic fibrosis. Occurrence of precipitating antibodies against pseudomonas aeruginosa in relation to the concentration of sixteen serum proteins and the clinical and radiographical status of the lungs." Acta Paediatr Scand 63(6): 843-8.
Kamogawa, A. and K. Kurahashi (1965). "Purification and properties of uridinediphosphate glucose pyrophosphorylase from Escherichia coli K12." J Biochem (Tokyo) 57(6): 758-65.
Komeda, Y., T. Icho, et al. (1977). "Effects of galU mutation on flagellar formation in Escherichia coli." J Bacteriol 129(2): 908-15.
Koplin, R., W. Arnold, et al. (1992). "Genetics of xanthan production in Xanthomonas campestris: the xanA and xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis." J Bacteriol 174(1): 191-9.
Lansing M. Prescott, John P. Harley, Donald A. Klein. Bacteria: The Proteobacteria, The McGraw-Hill Companies, Inc., 504-505, 2002
Leloir, L. F. (1964). "Nucleoside diphosphate sugars and saccharide synthesis." Biochem J 91(1): 1-8.
May, T. B., D. Shinabarger, et al. (1994). "Identification of amino acid residues involved in the activity of phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa." J Biol Chem 269(7): 4872-7.
May, T. B., D. Shinabarger, et al. (1991). "Alginate synthesis by Pseudomonas aeruginosa: a key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients." Clin Microbiol Rev 4(2): 191-206.
Nakae, T. and H. Nikaido (1971). "Multiple molecular forms of uridine diphosphate glucose pyrophosphorylase from Salmonella typhimurium. I. Catalytic properties of various forms." J Biol Chem 246(14): 4386-96.
Pannbacker, R. G. (1967). "Uridine diphosphoglucose biosynthesis during differentiation in the cellular slime mold. II. In vitro measurements." Biochemistry 6(5): 1287-93.
Reginald H. Garrett, Charles M. Grisham, Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway, Charlene Catlett Squibb, 755-757, 1999
Regni, C., P. A. Tipton, et al. (2002). "Crystal structure of PMM/PGM: an enzyme in the biosynthetic pathway of P. aeruginosa virulence factors." Structure (Camb) 10(2): 269-79.
Rehm, B. H. and S. Valla (1997). "Bacterial alginates: biosynthesis and applications." Appl Microbiol Biotechnol 48(3): 281-8.
Reynolds, H. Y., LEVINE, a s., WOOD, r. e., Ziierdt, C. H., Dale, D. C., and Pennington, J. E (1975). "Pseudomonas aeruginosa infections: persisting problems and current research to find new therapies." Ann Intern Med 82(6): 819-31.
Rocchetta, H. L., L. L. Burrows, et al. (1999). "Genetics of O-antigen biosynthesis in Pseudomonas aeruginosa." Microbiol Mol Biol Rev 63(3): 523-53.
Schulman, H. and E. P. Kennedy (1977). "Identification of UDP-glucose as an intermediate in the biosynthesis of the membrane-derived oligosaccharides of Escherichia coli." J Biol Chem 252(18): 6299-303.
Schwarzmann, S. W., J. L. Adler, et al. (1971). "Bacterial pneumonia during the Hong Kong influenza epidemic of 1968-1969." Arch Intern Med 127(6): 1037-41.
Shankar, S., R. W. Ye, et al. (1995). "Exopolysaccharide alginate synthesis in Pseudomonas aeruginosa: enzymology and regulation of gene expression." Adv Enzymol Relat Areas Mol Biol 70: 221-55.
Shinabarger, D., A. Berry, et al. (1991). "Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa." J Biol Chem 266(4): 2080-8.
Thorson, J. S., T. M. Kelly, et al. (1994). "Cloning, sequencing, and overexpression in Escherichia coli of the alpha-D-glucose-1-phosphate cytidylyltransferase gene isolated from Yersinia pseudotuberculosis." J Bacteriol 176(7): 1840-9.
Tsuboi, K. K., K. Fukunaga, et al. (1969). "Purification and specific kinetic properties of erythrocyte uridine diphosphate glucose pyrophosphorylase." J Biol Chem 244(3): 1008-15.
Weissborn, A. C., Q. Liu, et al. (1994). "UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme." J Bacteriol 176(9): 2611-8.
Wills, E. A., I. S. Roberts, et al. (2001). "Identification and characterization of the Cryptococcus neoformans phosphomannose isomerase-encoding gene, MAN1, and its impact on pathogenicity." Mol Microbiol 40(3): 610-20.
Wu, B., Y. Zhang, et al. (2002). "Bifunctional phosphomannose isomerase/GDP-D-mannose pyrophosphorylase is the point of control for GDP-D-mannose biosynthesis in Helicobacter pylori." FEBS Lett 519(1-3): 87-92.