C型凝集素家族成員可能參與了蚊子的免疫反應與生殖能力之間的生理平權過程，這種平權可能是由於資源不足而引起的，並且可能對潛在的生殖力產生重大影響。我們使用CRISPR / Cas9為C型凝集素家族的一個成員(GCTL-3) 建立了一純合子敲除的埃及伊蚊（Aedes aegypti）品系，用於研究GCTL-3 在蟲媒病毒感染及繁殖方面的代價之相關免疫反應與平衡作用。我們發現JAK / STAT，IMD，Toll和AMPs免疫途徑的多種成員被正向調控，並且改變了GCTL-3-/- 突變體的腸道共生菌群數量。與控制組相比，突變體還顯示出降低了DENV-2感染率，這可能是由於這些免疫途徑改變所致。
另一方面，突變體的壽命也比對照組短得多，並且生殖能力降低，這表示將資源轉移至免疫途徑可能導致生殖潛力降低。最後，我們發現dsRNA沉默了AMPs途徑的兩個特定成員，即Attacin和Gambicin，恢復了部分GCTL-3-/- 雌蚊的生育力和繁殖力，暗示這些成分對於依賴性C型凝集素在資源分配的過程中是極有影響力的。
蚊蟲資源分配策略不僅會影響蚊子的傳染性，並且還會影響產卵能力，因此對於媒介控制會產生重大影響。對其他C型凝集素家族成員作用的研究將進一步提高我們對免疫和生殖資源權衡的分子和遺傳機制的了解。

C-type lectin family members may be involved in the processes which determine resource distribution trade-offs between immune response and reproductive capability in mosquitoes. These trade-offs typically can arise due to insufficient resource availability, and can have significant impacts on reproductive potential. We used CRISPR/Cas9 to establish a homozygous KO Aedes aegypti line for one member of the C-type lectin family (GCTL-3) in order to investigate its’ role in balancing the costs associated with immune responses to arboviral infection and reproduction.
We found upregulation of multiple components of the JAK/STAT, IMD, Toll and AMPs immunological pathways and altered gut commensal microbiota populations in GCTL-3-/- mutants. Mutants also showed reductions in DENV-2 infection rates compared to controls, potentially due to these altered immune pathways. On the other hand, mutants also had significantly shorter lifespans than controls and diminished reproductive capability, suggesting that the diversion of resources to immune pathways may result in reduced reproductive potential. Finally, we found that dsRNA silencing of two specific components of the AMPs pathway, Attacin and Gambicin, led to a partial restoration of fertility and fecundity in GCTL-3-/- mutant females, implicating these components as highly influential components of C-type lectin dependent resource allocation processes.
Mosquito resource allocation strategies can have significant effects on vector control efforts as they influence not only mosquito infectivity but also egg laying capacity. Investigation of the role of other C-type lectin family members will further improve our knowledge of the molecular and genetic mechanisms underlying immunological and reproductive resource trade-offs.

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