生化法為常用農藥快篩方式之一，利用農藥抑制乙醯膽鹼酶進行檢測，雖然能用於市場即時檢測農藥殘留，但無法篩檢所有農藥。嘉磷塞(Glyphosate, GLY)為一種有機磷農藥，普遍施用於基因改良黃豆，由於GLY為乙醯膽鹼酶弱抑制劑，目前現行生化法無法適用於檢測GLY殘留。因此，本研究開發兩種檢測GLY殘留方法，期以在食品安全監測上具實質性助益。
本研究開發奈米金比色法(AuNPs colorimetry)檢測GLY，利用GLY和鉛離子之螯合效應，減弱鉛離子和AuNPs之間的靜電作用力，進而保留原本AuNPs顏色。比色法檢測結果顯示GLY濃度與AuNPs光吸收度具有良好負線性關係(R2 0.9964, LOD 0.0752 ppm)，AuNPs光吸收度隨GLY濃度上升而減弱。本研究針對AuNPs比色法亦開發微量農藥檢測儀器，以影像判讀比色法試片上GLY，可偵測到GLY濃度線性範圍介於0.1-1 ppm (R2 0.9645, LOD 0.025 ppm)。此外，本研究利用電化學方式開發分子印跡(Molecularly imprinted polymer, MIP)金電極，在電極表面修飾殼聚醣(Chitosan)，形成具有能吸附GLY之分子模腔，結合安培法(Amperometry, IT)檢測，可檢測GLY濃度線性範圍介於0.01-10 ppm (R2 0.8864, LOD 1.5 ppb)。利用MIP金電極-安培法檢測其他五種農藥，發現MIP金電極對於GLY具有專一性。本研究亦偵測黃豆樣本中GLY農藥殘留量，經水萃後，MIP金電極-安培法偵測結果呈現最佳線性濃度範圍為0.01-10 ppm (R2 0.9619, LOD 7.8 ppb)，涵蓋台灣衛生福利部食品藥物管理署(Taiwan Food and Drug Administration)法定GLY於各項農作物之容許殘留量(0.1-10 ppm)。本研究成功開發AuNPs比色法和MIP金電極-安培法能專一性檢測GLY，並且MIP金電極-安培法能檢測水萃黃豆樣本中GLY殘留，因此有助於提高農作物GLY殘留篩檢比率，促進食品安全。

The biochemical assay is one of the common screening methods for rapid pesticide detection by utilizing the attenuation of acetylcholinesterase. Although biochemical assay can be employed for real-time pesticide residue detection in the market, it is currently unable to screen for all commonly used pesticides. Glyphosate (GLY) is an organophosphorus pesticide frequently used in genetically modified soybeans. The current biochemical assay is not applicable for detecting residual GLY due to its weak inhibition of acetylcholinesterase. Therefore, this study developed two methods to detect residual GLY for food safety monitoring.
This study developed an AuNPs colorimetry method to detect GLY by the chelation effect of GLY with lead ions to weaken the electrostatic interaction between lead ions and AuNPs and preserve the original color of the AuNPs. The results of AuNPs colorimetry method indicated a strong negative linear relationship between GLY concentration and AuNPs absorbance (R2 0.9964, LOD 0.0752 ppm) with AuNPs absorbance decreasing as GLY concentration increased. In addition, this study created a trace pesticide detection instrument for the AuNPs colorimetry method, capable of conductong image-based analysis of GLY on the AuNPs colorimetry test strip (linear range 0.1-1 ppm, R2 0.9645, LOD 0.025 ppm). Furthermore, this study introduced a molecularly imprinted material (MIP) gold electrode using an electrochemical approach, with the surface modification of chitosan to create molecularly imprinted cavities capable of adsorbing GLY for the detection of GLY (linear range 0.01-10 ppm, R2 0.8864, LOD 1.5 ppb). The MIP gold electrode performed its specificity for GLY detection when tested against five other pesticides. The MIP gold electrode was employed to detect GLY residues in soybean samples after water extraction, revealing a linear range of 0.01-10 ppm (R2 0.9619, LOD 7.8 ppb), which complies with the permissible GLY residue limits of agriculture crops established by the Taiwan FDA (0.1-10 ppm). This study successfully developed the AuNPs colorimetric method and MIP gold electrode for the specific detection of GLY, and in particular, the MIP gold electrode was capable of detecting GLY residues in water-extracted soybean samples. Consequently, these developments contributed to enhancing the screening ratio for GLY residues in agricultural crops for promoting food safety

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