以光學影像技術觀察生物與醫學檢體組織，為瞭解生物組織功能與提供醫療判斷的重要工具。但由於生物組織為非勻相物質，其光散射性質，使組織的光穿透度不佳。因此，不論在實驗或者臨床醫學的運用上，樣本組織的切片觀察為主要的顯微影像的取得方式。但是切片會造成組織變形與切割破壞，無法保持完整的樣本結構。為了擺脫切片在光學影像技術上的限制，本實驗以小鼠胃組織為樣本，結合螢光染色與共軛焦顯微鏡技術，配合組織澄清試劑FocusClearTM使胃組織透明，提供了一個不需要使用切片機，即能進行胃組織觀察的方法，建立胃組織的三維顯微影像技術。
本實驗更進一步的應用所發展之三維胃組織顯微影像技術，觀察非類固醇類消炎藥(NSAIDs)所誘發的小鼠胃潰瘍結構，並與正常的胃組織結構比較，以期突破傳統平面病理切片觀察的限制。

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