本文旨在建立血管組織之建構平台，其設計重點放在光鉗、雷射導引以及微流道之整合。微流道內部分為血管內皮細胞建構區域、主流道以及中間流道。光鉗能在空間中移動細胞進行血管建構工作。雷射導引則能限制細胞在光軸方向移動，使細胞從主流道移動至建構區域。
系統運作流程如下。首先，內皮細胞經由注射式幫浦注入微流道內部，根據計算流體力學模擬，決定微流道內部的幾何尺寸，在不影響光鉗搬移細胞的前提下，使得微量的培養液能進入血管建構區域提供細胞養分。其次，細胞經由雷射導引通過中間流道，到達細胞建構區域。之後，光鉗可在中間流道的末端取得細胞，移動細胞至特定位置，進行血管建構工程。
鏡面驅動式光鉗之光路參數初階設計是基於近軸公式，吾人使用光學軟體對此參數進行最佳化。經過最佳化後，光鉗的移動範圍可包括70%的物鏡視野。對於N.A.值1.25的油鏡而言，光鉗工作範圍約為直徑70 ~ 80um的圓形。

The concept of the blood capillary construction system is based on three modules: optical tweezers, laser guidance and the fabrication of micro channels. Inside of micro channels can be divided into three parts, capillary construction area, main channel and sub channels.
The working process of the system is as follows. First, the endothelial cells were stored in a syringe and pumped into main channel. With computational fluid dynamics simulation, the geometry of micro channels was designed to allow sufficient nutrition entering the constructive region so as to keep the cell vitality but would not let the flow disturb the process. Second, the cells were guided into sub channel, lined up and flow through the channel toward construction area by laser guidance. Finally, optical tweezers trapped cells at the end of the sub channel and put them at the proper place to form a piece of blood capillary.
Optical tweezers were driven by tilting the mirror. Parameters calculated from the thin-lens formula were used as initial conditions and then optimized by optics simulation software. After optimization, the working range of the optical tweezers could cover 70% field of view of the microscope objective. For a N.A.=1.25 oil immersion-type microscope objective, the working area is a circle which diameter of 70 ~ 80um

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