過去的研究已經發現心臟毒素A3在細胞膜中形成孔洞時，腦硫脂扮演著重要的角色（王佳蕙，2005，博士論文）。因此本論文首先利用（一）本質性螢光光譜以及恆溫滴定熱卡計實驗，研究混有腦硫脂的微胞結合心臟毒素A3和A5的作用模式，（二）利用核磁共振光譜實驗，研究腦硫脂的頭部醣基構形-半乳醣衍生物結合心臟毒素A3與A5的可能位置。結果發現混有腦硫脂的微胞結合心臟毒素A3和A5的作用模式互不相同；心臟毒素A3與半乳醣衍生物的結合位置為蛋白的核心區域，而心臟毒素A5的結合位置則靠近指環Ⅰ的位置。以上研究可以說明，心臟毒素A3與A5在細胞脂質膜中與腦硫脂的作用模式不同，且在心臟毒素A3進入細胞膜的初期，腦硫脂的頭部醣基構形會插入心臟毒素A3的核心區域，進行結合後再引導心臟毒素A3進入細胞膜，但是心臟毒素A5則僅在細胞膜表面結合。這個結果可以解釋為何心臟毒素A5不易在生物細胞膜中形成孔洞導致細胞死亡。

It has recently been shown that sulfatide plays an important role in pore-forming process of CTX A3 on cell membrane. In order to understand the details of CTX – sulfatide interaction, we employed intrinsic fluorescence and isothermal titration calorimeter (ITC) to study the binding mode of CTX A3 and A5 to micelles containing sulfatide. We also applied nuclear magnetic resonance to investigate the possible binding CTX site of galactose derivative(the sulfogalactoyl headgroup moiety of sulfatide). Our results suggest that the binding mode of CTX A3 with sulfatide and its headgroup mimetics is different from that of CTX A5. Specifically,sulfatide headgroup and its derivative binds to CTX A3 at the core region whereas that binds to CTX A5 near loop I region. The different mode of sulfatide interaction with CTX A3 and CTX A5 provides an explanation on why CTX A5 could not form pore in cell membranes.

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