白點病毒(White Spot Syndrome Virus)具有廣泛宿主的特性，除了是造成養殖蝦類高死亡率的白點病的病因，對於其他種類的水生甲殼類亦導致高死亡率的肇因。截至目前為止，仍無有效防治白點病的方法，如何解決白點病造成的損害成乃為各國水產研究單位刻不容緩的課題。WSSV的套膜蛋白質VP28 (envelop protein VP28)在感染蝦子的過程中扮演重要的角色，目前發現抗VP28的抗體具有中和或延遲WSSV感染蝦子的功效。因此本研究發展利用以注射及餵食的方式，將外來的中和病毒的抗體到蝦子體內，以補足蝦子免疫系統的不足。雖然一般均認為蝦子的免疫系統不具有如脊椎動物般的cell-mediated adaptive immunity system；但是為開發使用抗VP28抗體中和WSSV的實際應用，即施用抗VP28的抗體並維持其在蝦子體內維持的穩定濃度來防治WSSV的感染。由於PEGyaltion後的蛋白質在高等生物體內能延遲代謝的速度，相對地更具療效。於是本研究利用PEGyaltion對蛋白質的修飾以達到目的，本研究將具有特異性的抗VP28的單株抗體(□VP28)經過PEGyaltion後，以注射與口服的方式施用於蝦子以評估被動免疫療法(passive immunity therapy)應用在蝦子的可能性。比較直接注射經PEGyaltion修飾的□VP28與未經過修飾的□VP28的作用，顯示PEGyaltion的確能延長□VP28在蝦子體內存留約長兩倍的時間；除此之外，本研究亦是首次證實經PEGyaltion修飾後的單株抗體在無脊椎動物體內延長其生物功能(biological function)。至於餵食的試驗方面，則不論PEGyaltion修飾或未經修飾的□VP28都無法通過消化道的考驗進入到循環系統。綜合而言，本研究顯示將PEGyaltion修飾後的□VP28注射到蝦子體內為具有開發潛力的防治白點病新穎方法。

Besides high mortal rate to cultured shrimp, White Spot Syndrome Virus (WSSV) also causes high mortality to other species of aquatic crustacean. As a result, there is an urgent need to find the solutions for epidemic of WSSV. From previous studies demonstrate that VP28, an envelop protein of WSSV, is critical for WSSV infection; and the infection of WSSV could be neutralized or attenuated through the existence of anti-VP28 antibodies within shrimp body. However, the concentration of anti-VP28 antibodies within shrimp must have to maintain a stable and efficacious level for inhibition of WSSV infection. Actually, PEGylated protein can sustain more stable concentration within the body of vertebrate organism and appeared to be more efficacious of its biological functions. The specific aims of this study is tried to maintain the concentration and duration of anti-VP28 antibodies within shrimp body through PEGylation modification. After PEGyaltion of anti-VP28 antibodies (□VP28), antibodies were introduced into shrimp bodies through either intramuscular injection or oral feeding. From the results, PEGylaton provided □VP28 twice times of duration, as comparing to non-PEGylated. In fact, this result is the first report that monoclonal antibodies could extend the duration within the body of invertebrate organism through PEGylation modification. As oral feeding, neither PEGylated nor non-PEGylated □VP28 could not survive from the GI track. Overall, the □VP28 might be a practical treatment for inhibition of WSSV infection after PEGylation.

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