Tumor suppressor p53 is an essential component to maintain the genomic
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299-308 (LPPGSTKRAL) of p53 is indispensable to both the excision activity and the
p53: XPB interaction. In this study, the two positive charged amino acid residues,
K305 and R306 of positions 299-308 were substituted with alanine, individually or
simultaneously, to test the importance of electrostatic contribution in this regard.
Results of the study show that the excision activity of nuclear extract and the
recruitment of XPB and RPA to UV-induced DNA damage sites was reduced in the
order of K305A≧ R306A/K305A> R306A. RPA is a single- DNA binding protein, it
is recruited to the DNA damage sites during NER pathway once the duplex DNA of
the sites are unwound by helicase XPB/XPD of TFIIH complex. This study
demonstrates the importance of electrostatic contribution to p53: XPB interaction in
NER.

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