血管新生在傷口修復、器官移植，以及腫瘤生長和轉移，都扮演著非常重要的角色。然而，目前許多促血管生長因子或是抗血管新生的藥物篩選研究，都是在二維的單層細胞培養條件下進行，無法真正模擬體內組織的微環境。最近微流體技術的長足發展提供細胞三維培養良好的微環境，可做為體外血管新生研究的平台。然而目前微流體技術常有設計複雜、操作困難等缺點。此外，在微流體系統培養內皮細胞的過程，給予適當流速以進行培養液的連續灌流仍相當困難。在本研究中，我們設計了三種微流體系統以建立體外三維血管新生模式，分別為PEG-DA微結構、PDMS微結構和無任何微結構之微流體晶片。接著將製作好的微流體晶片建立三維細胞培養微環境，包括將腫瘤類球體和內皮細胞進行共培養，提供不同流速、垂直或水平放置微流體晶片，以及給予細胞不同濃度的血管內皮生長因子，探討不同環境變因對於血管新生的影響。實驗結果顯示，在無結構之微流體晶片中，內皮細胞可形成類似血管的管狀結構，並可培養至第5天。在垂直微流體晶片中給予2 μL/min的流速，能在本研究設計之微流體晶片中建立一個最適當的血管新生環境。在模擬體內腫瘤引起血管新生的研究中，將腫瘤類球體和內皮細胞進行共培養，更能觀察到內皮細胞被誘導朝腫瘤類球體的方向生長遷移及進行血管新生的情形。此外，光學同調斷層掃描能對於在三維環境中的腫瘤類球體進行斷層掃描之影像擷取。因此，微流體晶片合併光學同調斷層掃描能提供一個完整平台，未來對於生物學上的分析及藥物篩選，有相當大的幫助。

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