本篇論文中，描述如何利用LB film成膜儀(Langmuir-Blodgett Troughs)在奈微米孔洞上有效率地形成自組裝雙層膜，並結合原子力顯微術(Atomic Force Microscopy, AFM)進行掃描分析，以期進一步建立起觀測膜蛋白的檢測平台。
研究中，使用E-Beam 微影和濕式蝕刻製程在Si晶片上的Si3N4薄膜上打出奈微米陣列孔洞(pores)，並挖空Si基材的晶片結構，來作為形成雙脂層薄膜的支撐。然後再以接觸式的原子力顯微鏡掃瞄出奈微米孔洞的成膜情形與孔洞直徑的量測。接著再以Langmuir- Blodgett 法於孔洞結構的Si3N4薄膜上形成雙脂層薄膜，同時比較不同參數(膽固醇添加物、稀釋程度、分子有無雙鍵結構、成膜溫度等)條件下，對於LB film成膜情形的影響，藉由各項參數所反映出的成膜臨界壓力值，來分析液面上單分子膜層的緊密排列情形，接著再利用原子力顯微鏡的幫助來掃描成膜晶片中央的孔洞是否有雙層膜形成，藉此來比較成膜的成功與否。如此一來便做出似生物膜狀態的結構，再以此架構作為膜蛋白檢測平台。
實驗結果顯示，成膜臨界壓力值與膽固醇的添加、較高的稀釋度、無雙鍵油脂分子結構及較高的成膜溫度，有正向的關係呈現。

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