本論文將以各種不同的實驗架構和量測方法，應用於表面電漿共振感測器的研究與發展上。其實驗架構分為強度量測架構、共光程外差干涉架構、以及改良式共光程外差干涉架構，而量測方法也分為傳統的強度量測法、干涉強度量測法、以及干涉相位量測法。其中改良式外差干涉術，為我們最新提出的量測方式，是在共光程外差干涉儀中，加入兩個四分之一波片，藉此增加我們量測折射率的靈敏度。
我們將對各個實驗架構，作理論的推導和圖形的模擬，並配合實驗量測數據，以比較各系統和量測方法之間的差異及好壞，之後並使用干涉架構來作蛋白質與蛋白質的結合實驗，期望未來能將此架構應用於生醫量測上。本篇論文證實了我們提出的改良式外差干涉術量測相位的方式，應用於表面電漿共振感測器的量測上，是目前最靈敏的量測架構和方法，其理論的折射率靈敏度估算可達4×10-9RIU（refractive index unit），而實驗的數值也可達3.4×10-6RIU。至於蛋白質的量測方面，最低感測濃度可達0.3μg/ml。

The investigation of surface plasmon resonance by several measurement systems based on the measurement of intensity or the measurement of phase are described in this dissertation. The measurement systems consist of (1) an optical intensity-based system, (2) an intensity-based interferometric system, (3) a heterodyne interferometric system, and (4) a novel common-path heterodyne interferometric system. The former two are based on the measurement of intensity while the others are based one the measurement of phase. Among them, the novel common-path heterodyne interferometric system, which is built with a pair of quarter waveplates to improve the measurement sensitivity, is my original design.
The surface plasmon resonance for each measurement system is theoretical and experiment verified. The results from each system are used to make a comparism. In effect, the most sensitive one is my original design, the novel common-path heterodyne interferomatic system. The measurement resolution of refractive index for this system is theoretically estimated to be 4×10-9RIU(refractive index unit). An experiment result of 3.4×10-6RIU has been achieved by this study. Furthermore, a protein-protein interaction experiment is also included. So far, the detection limit for protein of 0.3μg/ml has been obtained. Both the measurement resolution and the detection limit for protein have a great space to improve in the near future.

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