過去大家所熟知的日本絨螯蟹 ( Eriocheir japonica ( de Haan, 1835 ) ) 在分類上屬十足目 ( Decapoda ) ，方蟹科 ( Grapsidae ) 。其分布北自庫頁島經朝鮮半島東岸、日本，南至中國大陸及台灣，包含諸多不連續的島嶼，且為台灣原生的絨螯蟹之一。現今在台灣主要分布於台中的濁水溪以北至宜蘭的白米溪，並於宜蘭的梗枋溪、新城溪與白米溪發現與台灣扁絨螯蟹 ( Platyeriocheir formosa ( Chan, Hung and Yu, 1995 ) ) 有共域的現象。本種在秋冬產卵的習性與台灣扁絨螯蟹於春夏之際產卵不同，除了具有相當高的經濟價值之外，在學術研究上更有重要的地位。
本研究利用 PCR 與定序等技術，以粒線體 DNA 細胞色素氧化酶 I ( Mitochondrial cytochrome oxidase I, COI ) 序列前段 555 bp作為分子標記 ( Molecular marker ) ，利用此分子標記探討台灣本島內日本絨螯蟹的遺傳多樣性與族群遺傳結構，總共分析了台灣與琉球沖繩地區 61 隻的日本絨螯蟹個體。結果在序列鹼基對比例方面，發現呈現 A – T rich 的現象，並加入從 GenBank 所獲得的四條序列，共得到 23 個基因型。由 DnaSP version 4.0 計算單倍基因多樣性指數 ( Haplotype diversity, h ) 與核苷酸多樣性指數 ( Nucleotide diversity, π ) 得知高的單倍體基因多樣性指數與低的核苷酸多樣性指數的結果，再利用 Tajima’s D 和 Fu and Li’s D 的中性檢定結果，顯示顯示族群有擴張的現象，因此，推測日本絨螯蟹族群曾經歷過瓶頸效應，而後快速擴張。以 Neighbor – joining 法進行親緣關係數的建構，顯示東亞地區分成日本、琉球沖繩、與台灣及中國大陸等三大群，其 bootstrap 值皆高於 90 。推測，真正的日本絨螯蟹應僅是產於日本本島的族群，而台灣及中國大陸的族群，實應歸屬於合浦絨螯蟹 ( E. hepuensis ) ，琉球沖繩地區的絨螯蟹則應為一新種，其學名暫定為 E. sp. nov. 。造成日本、琉球沖繩與台灣及中國大陸分成三群的原因，可能是受到 Kerama 與 Tokara 兩海槽的影響。根據 NJ Tree 分群的結果，建議將其分成三個演化有效單位 ( ESU ) 與管理單位 ( MU ) 進行保育管理。台灣島內的三個小地理區的族群呈現低遺傳分化與高基因交流的現象，推測，沿岸流可能是造成各族群交流的主要原因之一。

The Japanese mitten crab, Eriocheir japonica (de Haan, 1835), belonged to Decapoda, Grapsidae, is distributed in eastern Asia including Sakhalin, the eastern Korean Peninsula, Japan, China and Taiwan. In Taiwan, it is distributed in the northwest of Taiwan, from Juo-Shuei River in the central Taiwan to Bai-Mi River in Yilan County. This species and the Taiwanese mitten crab, Platyeriocheir formosa, are sympatric in Keng-Fang River, Shin-Cheng River and Bai-Mi River, which are located in Yilan. The reproductive behaviors are different between the two mitten crabs in Taiwan. The Japanese mitten crab migrated and reproduced in autumn and winter but the Taiwanese in spring and summer.
Mitochondrial COI gene was used to be a molecular marker to reveal the population genetics of E. japonica in Taiwan. Sixty-one specimens were analyzed and a partial fragment of COI gene, whose size is 555 bps in length, was obtained. Twenty-three haplotypes were obtained including 3 classified from four sequences in GenBank. The high haplotype but low nucleotide diversity of the Japanese mitten and the results of Tajima’s D and Fu and Li’s D test were indicated that the populations of Japanese mitten crab have experienced bottleneck effect and population expansion event in the past. The NJ tree of the mitten crabs have shown that the Japanese mitten crabs are divided into three major groups with high bootstrap, which are group Japan, group Okinawa and group Taiwan and China. This division may result from the affections of Kerama and Tokara gap in western pacific. We hypothesize that there might be three species existed in Japanese mitten crabs. E. japonica could indicate the populations in Japan. The populations in Okinawa could be a new specie ( E. sp. nov. ) and the populations in Taiwan and China could be the members of E. hepuensis. In order to protect the populations of Japanese mitten crabs in Taiwan, three evolutionarily significant unit ( ESU ) and management unit ( MU ) should be proposed. The low genetic diversity and high frequent gene flow among the populations of Japanese mitten crabs in Taiwan could be influenced by the coastal current.

王昱人。 1997 。台灣鉤吻鮭與日本櫻花鉤吻鮭遺傳多樣性之研究，國立清華大學生物資訊與結構生物研究所碩士論文， 65 頁。

孔曉瑜、喻子牛、劉亞軍、高天翔、武雲飛。 2001 。中華絨螯蟹與日本絨螯蟹粒線體 COI 基因片段的序列比較研究，青島海洋大學學報， 31 ( 6 )： 861 ~ 866 。

李孟仙、曾輝。 2000 。合浦絨螯蟹 Eriocheir hepuensis 的形態學研究。浙江海洋學院學報 ( 自然科學版 ) 。 19(4)： 327~332 。

李春生。 1989 。大陸與台灣的地理淵源，台灣動物地理淵源研討會專集， 1 ~ 11 。

李思發、鄒曙明。 1999 。中國大陸沿海六水系絨螯蟹 ( 中華絨螯蟹和日本絨螯蟹 ) 群體親緣關係： RAPD 指紋標記。水產學報。 23(4)： 325~330 。

李榮祥。 2001 。台灣賞蟹情報，大樹文化， 174 頁。

李錦地、郭錦洛、王松賓。 1977 。以底棲大型無脊椎動物及魚類之群落變異指數和指標生物評估台灣河川水質，台灣省水污染防治所研究報告， 1： 1 ~ 44 。

周明鎮、張彌曼、陳宜瑜、朱敏。 1996 。隔離分化生物地理學譯文集，中國大百科全書出版社， 326 頁。

林宏都。 1998 。台灣地區台灣鏟頜魚族群遺傳結構之研究，國立台灣師範大學生物研究所碩士論文， 95 頁。

林朝棨、周瑞燉。 1974 。台灣地質，台灣省文獻委員會， 450 頁。

邱高峰、徐巧婷、王麗卿、范崢、陳雪菁。 2001 。四種絨螯蟹分子分類與系統發育，動物學報， 47 ( 6 )： 640 ~ 647 。

施習德。 2002 。甲殼類的分子生態學，科學月刊， 388： 307 ~ 314 。

施雅風、崔之久、李吉均。 1989 。中國東部第四紀冰川與環境問題， 450 頁。

柳榗。 1966 。台灣松柏類植物地理之研究，林業試驗所報告， 122： 1 ~ 33 。

柳榗。 1989 。台灣生物地理概說，台灣動物地理淵源研討會專集， 13 ~ 23 。

洪明仕。 1993 。南澳南溪直額絨螯蟹族群動態及生物學之研究，國立海洋大學漁業學系碩士論文， 67 頁。

洪龍華。 2002 。利用細胞核核糖體 DNA 基因之 ITS 序列和轉譯延長因子重建台灣沼緣蜉蝣 ( Cloeon marginale Hagen ) 的親緣地理，國立成功大學生物學研究所碩士論文， 73 頁。

胡忠恆。 1995 。台灣地質概論，台灣貝類化石誌第四卷第十九冊， 112 頁。

唐伯平。 2000 。絨螯蟹屬的分子系統發生學。南京師範大學理學博士學位論文。 99 頁。

高天翔、任一平、張秀梅。 2000 。日本絨螯蟹粒線體 DNA 序列研究 II. 16S rRNA ，青島海洋大學學報， 30 ( 3 )： 482-486 。

高天翔、張秀梅、樓東、張軍生。 1999 。日本絨螯蟹太平洋群體和日本海群體 12S rRNA 序列比較研究。浙江海洋學院學報 ( 自然科學版 ) 。 18(4)：275~278 。

高志千、周開亞。 1998 。中華絨螯蟹遺傳變異的 RAPD 分析。生物多樣性。 6(3)：186~190 。

孫紅英、王光躍、張代臻、周開亞。 2005 。中華絨螯蟹與合浦絨螯蟹兩地理亞種的線粒體 DNA 序列變異。動物學報。 51(5)：862~866 。

孫紅英、周開亞、陳健健、楊光。 2002 。中國大陸絨螯蟹線粒體 16S rRNA 序列變異與分子鑑定標記。自然科學進展。 12(5)：485~490 。

孫紅英、周開亞、楊小軍。 2003 。從線粒體 16S rRNA 序列探討絨螯蟹類的系統發生關係，動物學報， 49 ( 5 )： 593 ~ 599 。

殷秀琴、侯威岭、李貞。 2004 。生物地理學，高等教育出版社， 374 頁。

許桂菁、蔡昆展、邵廣昭、王建平、林弘都。 2005 。大眼華鯿親緣地理與族群遺傳結構之研究，生物學報， 40 ( 2 )： 58 ~ 67 。

許桂菁。 2007 。後冰河時期南中國中華花鰍 ( Cobitis sinensis ) 之遷移路徑，國立成功大學生物學研究所博士論文， 134 頁。

莊士鋒。 2003 。以分子遺傳探討台灣產岩瓷蟹類之類緣關係，國立中山大學海洋資源研究所碩士論文， 118 頁。

陳正祥。 1957 。氣候之分類與分區，台灣大學林業叢刊第 7 號， 174 頁。

陳均輝。 1998 。太魯閣國家公園砂卡礑溪台灣絨螯蟹之生物學研究，國立海洋大學海洋生物研究所碩士論文， 55 頁。

陳溫柔、施志昀、鄭金華、徐芝敏。 2001 。高屏地區水系之淡水蟹類，自然保育季刊， 36： 42 ~ 47 。

陳蕙蓮、孫海寶。 2002 。中國動物志無脊椎動物第三十卷節肢動物門甲殼動物亞門短尾次目海洋低等蟹類，科學出版社， 597 頁。

堵南山。 2002 。關於絨螯蟹屬的分類，水產科技情報， 29 ( 1 )： 10 ~ 12 。

黃世彬。 2005 。中國韓江流域吻鰕虎魚 ( Rhinogobius ) 的分類研究，國立屏東科技大學野生動物保育研究所碩士論文， 85 頁。

黃鈞漢。 2003 。以 mtDNA D – loop 探討艾氏樹蛙 ( 溪頭地區 ) 之族群遺傳結構，國立彰化師範大學生物學系碩士論文， 64 頁。

劉名允。 2006 。沼蝦屬分子系統分類及台灣的粗糙沼蝦親緣地理和族群遺傳探討，國立清華大學生物資訊與結構生物研究所博士論文， 73 頁。

詹存勇、楊穎堅、林勝豐。 1996 。黑潮於台灣東北海域的季節性入侵，國科會海洋研究計畫成果發表會論文摘要集， 13 ~ 19 頁。

鄭光膺。 1991 。黃海第四紀地質，科學出版社， 179 頁。

鄭明修。 1998 。墾丁國家公園的 ─ 蝦兵蟹將，墾丁國家公園管理處員工消費合作社， 133 頁。

鄭清海、林鴻仁、王美鳳、詹見平。 1998 。賞蟹之旅 ─ 台中縣海邊常見的螃蟹，台中縣永安國民小學， 92 頁。

賴弘智、施志昀、游祥平。 1986 。室內孵化之日本絨螯蟹 ( Eriocheir japonica De Haan ) 幼苗變態研究，台灣水產學會刊， 13 ( 2 ) ： 12 ~ 21 。

賴景陽。 1989 。台灣的哺乳動物化石紀錄，台灣動物地理淵源研討會專集， 25 ~ 48 。

蕭程友。 2007 。台灣絨螯蟹 ( Eriocheir formosa ) 遺傳多樣性之研究，國立清華大學生物資訊與結構生物研究所碩士論文， 57 頁。

薛攀文。 2000 。蟹類大眼幼蟲在潮水誘發之返岸運送中的回歸模式研究報告。國科會研究報告 NSC89-2611-B005-002 。

戴愛雲、楊思諒、宋玉枝、陳國孝。 1986 。中國海洋蟹類，海洋出版社， 642 頁。

戴愛雲。 1988 。絨螯蟹屬支序分類學的初步分析，動物分類學報， 13 ( 1 )： 22 ~ 26 。

Avise, J. C., J. Amold, R. M. Ball, E. Bermingham, T. Lamb, J. E. Neigel, C. A. Reed and N. C. Saunder. 1987. Intraspecific phylogeography: the mitochrondrial DNA bridge between population genetics and systematics. Annual Review of Ecology and Systematics. 18: 489-522.

Avise, J. C. 1989. Gene trees and phylogenetic approach to population biology. Evolution. 43: 1192-1208.

Avise, J. C. 1994. Molecular markers, natural history and evolution. Sinauer Associates. Chapman and Hall. New York. 684 pages.

Bermingham, E. and A. P. Martin. 1998. Comparative mtDNA phylogeography of neotropical freshwater fish: testing shared history of infer the evolutionary landscape of lower Central Americal. Molecular Evolution. 7: 499-517.

Bernachez, L. and C. C. Wilson. 1998. Comparative phylogeography of Nearctic and Palearctic fishes. Molecular Ecology. 7: 431-452.

Brown, R. P., R. S. Thorpe and M. Baez. 1991. Parallel within-island microevolution of lizard on neighbouring island. Nature. 352: 60-62.

Brown, J. H. and M. V. Lomolino. 1998. Biogeography. Sinauer Associates. Sunderland. Massachusetts. 845 pages.

Bruyn, M. D., J. C. Wilson and P. B. Mather. 2004. Reconciling geography and genealogy: phylogeography of giant freshwater prawns from the Lake Carpentaria region. Molecular Ecology. 13: 3515-3526.

Bucklin, A., B. W. Frost, J. Bradford-Grieve, L. D. Allen and N. J. Copley. 2003. Molecular systematic and phylogenetic assessment of 34 calanoid copepod species of the Calanidae and Clausocalanidae. Marine Biology. 142: 333-343.

Byrne, R. 1980. Man and the variable vulnerability of island life: a study of recent vegetation change in the Bahamas. Atoll Research. Washington. 200 pages.

Cassone, B. J. and E. G. Boulding. 2006. Genetic structure and phylogeography of the line shore crab, Pachygrapsus crassipes, along the northeastern and western Pacific coasts. Marine biology. 149: 213-226.

Castilla, A. M., V. Fernández-pedrosa, T. Backeljau, A. gonzález, A. Latorre and A. Moya. 1998. Conservation genetics of insular Podarcis lizards using partial cytochrome b sequences. Molecular Ecology. 7: 1407-1411.

Chan, T. Y., M. S. Hung and H. P. Yu. 1995. Identity of Eriocheir recta ( Stimpson, 1958 ) ( Decapoda: Brachyura ), with description of a new mitten crab from Taiwan. Journal of Crustacean Biology. 15(2): 301-308.

Chu, K. H., J. G. Tong and T. Y. Chan. 1999. Mitochondrial cytochrome oxidase I sequence divergence in some Chinese species of Charybdis ( Crustacea: Decapoda: Portunidae ). Biochemical Systematics and Ecology. 27(5): 461-468.

Chu, K. H., H. Y. Ho, C. P. Li and T. Y. Chan. 2003. Molecular phylogenetics of the mitten crab species in Eriocheir, sensu lata ( Brachyura: Grapsidae ). Journal of Crustacean Biology. 23(3): 738-746.

Clark, P. F., I. M. A. Sahib and M. S. A. Asadi. 2006. The first record of Eriocheir sinensis H. Milne Edward, 1853 ( Crustacea: Brachyura: Varunidae ) from the Basrah Area of Southern Iraq. Aquatic Invasions. 1(2): 51-54.

Crandall, K. A. and J. F. Fitzpatrick, Jr. 1996. Crayfish molecular systematics: using a combination of procedures to estimate phylogeny. Systematic Biology. 45(1): 1-26.

Dai, A. Y. 1991. Studies on the subspecies differentiation of the genus Eriocheir ( Decapoda: Brachyura ). Science Treat System Evolution Zoology. 1: 61-71.

Daniels, S. R., B. A. Stewart and P. A. Cook. 2002. Congruent patterns of genetic variation in a burrowing freshwater crab revealed by allozymes and mt DNA sequence analysis. Hydrobiologia. 468: 171-179.

Daniels, S. R. 2003. Examining the genetic structure among populations of the common cape river crab Potamonautes perlatus from river systems in south Africa reveals hydrographic boundaries. Journal of Crustacean Biology. 23(4): 936-950.

DeVries, M. C. and R. B. Forward. 1989. Rhythms in larval release of the sublittoral crab Neopanope sayi and the supralittoral crab Sesarma cinereum ( Decapoda: Brachyura ) . Marine Biology. 100: 241-248.

Dlayton, D. A. 1982. Replication of animal mitochondrial DNA. Cell. 28: 693-705.

Douglas, M. R., P. C. Brunner and M. E. Douglas. 2003. Drought in an evolutionary context: molecular variability in Flannelmouth Sucker ( Catostomus latipinnis ) from the Colorado River Basin of western North America. Freshwater Biology. 48: 1254-1273.

Erik, G. M., D. Nicole, O. S. Mario, M. Jean-Claude and M. Monique. 1993. Mitochondrial 16S-rRNA gene of two species of shrimps: sequence variability and secondary structure. Crustaceana. 65(3): 279-286.

Excoffier, L. 1993. Analysis of molecular variance. Version 1.55. Genetics and Biometry Laboratory, University of Geneva.

Excoffier, L. and P. E. Smouse. 1994. Using allele frequencies and geographic subdivision to reconstruct gene trees within a species: molecular variance parsimony. Genetics. 136: 343-359.

Excoffier, L., P. E. Smouse and J. M. Quattro. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics. 131: 479-491.

Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 39: 783-791.

Fu, X. Y. and W. H. Li. 1993. Statical tests of neutrality of mutations. Genetics. 133: 693-709.

Futuyma, D. J. 1997. Evolutionary biology. Sinauer Associates. NewYork. 763 pages.

Futuyma, D. J. 2005. Evolution. Sinauer Associates. NewYork. 543 pages.

García-Rodríguez, F. J. and R. Perez-Enriquez. 2006. Genetic differentiation of the California spiny lobster Panulirus interruptus ( Randall, 1840 ) along the coast of the Baja California Peninsula, Mexico. Marine Biology. 148: 621-629.

Grant, W. and B. W. Bowen. 1998. Shallow population histories in deep evolutionary lineages of marine fishes: insights from sardines and anchovies and lessons for conservation. Journal of Heredity. 89: 415-426.

Guo, J. Y., N. K. Ng, A. Y. Dai and P. K. L. Ng. 1997. The taxonomy of three commercially important species of mitten crabs of the genus Eriocheir ( de Haan 1835 ) ( Crustacea: Decapoda: Brachyura: Grapsidae ). The Ruffles Bulletin of Zoology. 45(2): 445-476.

Harrison, M. K. and B. J. Crespi. 1999. Phylogenetics of Cancer crabs ( Crustacea: Decapoda: Brachyura ). Molecular Phylogenetics and Evolution. 12(2): 186-199.

Harrison, J. S. 2004. Evolution, biogeography, and the utility of mitochondrial 16S and COI genes in phylogenetic analysis of the crab genus Austinixa ( Decapoda: Pinnotheridae ). Molecular Phylogenetics and Evolution. 30: 743-754.

Hashimoto, M. 1899. Geology of Japan. Springer. Tokyo. 300 pages.

Herborg, L. M., M. G. Bentley, A. S. Clare and K. S. Last. 2006. Mating behaviour and chemical communication in the invasive Chinese mitten crab Eriocheir sinensis. Journal of Experimental Marine Biology and Ecology. 329: 1-10.

Hey, J. and R. Nielsen. 2004. Multilocus methods for estimatiog population sizes, migration rates and divergence time, with applications to the divergence of Drosophila pseudoobscura and D. persimilis. Genetics. 167: 747-760.

Hillis, D. M. and J. J. Bull. 1993. An empirical test of bootstrapping as a method assessing confidence in phylogenetic analysis. System Biology. 41: 182-192.

Ho, P. H., H. P. Yu and P. K. L. Ng. 2001. The Indo-Pacific Pilumnidae XIV. On a new species of Actumnus ( Crustacea: Decapoda: Brachyura ) from Taiwan. Proceedings of the Biological society of Washington. 114(1): 145-151.

Hudson, R. R., M. Slatkin and W. P. Maddison. 1992. Estimation of levels of gene flow from DNA sequence data. Genetics. 132: 583-589.

Jukes, T. H. and C. R. Cantor. 1969. Evolution of protein molecules. In: Mammalian Protein Metabolism ( ed. Munroled HN ). Academic Press. New York. 31-132.

Kawane, M., K. Wada, J. Kitaura and K. Watanabe. 2005. Taxonomic re-examination of the two camptandriid crab species Deriatonotus japonicus ( Sakai, 1934 ) and D. tondensis Sakai, 1983, and genetic differentiation among their local populations. Journal of Natural History. 39(45): 3903-3918.

Khoo J. 2007. Finding the new species of Macrobrachium by using mitochondrial DNA and morphology. Institute of Bioinformatics and Structural Biology. 58 pages.

Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. 16: 111-120.

Kimura, T., I. Hayami and S. Yoshida. 1991. Geology of Japan. University of Tokyo Press. Tokyo. 320 pages.

Kobayashi, S. and S. Matsuura. 1995. Population structure of the Japanese mitten crab Eriocheir japonicus De Haan – clinal variations in size of maturity. Crustacean Research. 24: 128-136.

Kobayashi, S. 1999. Dimorphism in adult male Japanese mitten crab Eriocheir japonica De Haan . Crustacean Research. 28: 24-36

Komai, T., I. Yamasaki, S. Kobayashi, T. Yamamoto and S. Watanabe. 2006. Eriocheir ogasawaraensis Komai, a new species of mitten crab ( Crustacea: Decapoda: Brachyura: Varunidae ) from the Ogasawaea Island, Japan, with note on the systematics of Eriocheir De Haan, 1835. Zootaxa. 1168: 1-20.

Kumar, S. K., I. B. Tamura and N. M. Jakobsen. 2001. MEGA2: molecular evolutionary genetic analysis software. Bioinformatics. 17: 1244-1245.

Lee, J. W., L. Jiang, Y. C. Su and I. M. Tso. 2004. Is central mountain range a geographic barrier to the giant wood spider Nephila pilipes ( Araneae : Tetragnathidae ) in Taiwan? A population genetic approach. Zoological Studies. 43(1): 112-122.

Lee, T. H., N. Naitoh and F. Yamazaki. 2004. Chromosome studies on the mitten crabs Eriocheir japonica and E. sinensis. Fisheries Science. 70: 211-214.

Li, W. H. and A. Zharkikh. 1995. A statistical test of DNA phylogenies. Systematic Biology. 44: 49-63.

Lin, S. M., C. A. Chen and K. Y. Lue. 2002. Molecular phylogeny and biogeography of the grass lizards genus Takydromus (Reptilia: Lacertidae) of East Asia. Molecular Phylogenetics and Evolution. 22(2): 276-288.

Lu, R. H., T. Qiu, C. M. Xiang, H. Xie and J. Zhang. 2000. RAPD and AFLP techniques for the analysis of genetic relationships in two genera of Decapoda. Crustaceana. 73(9): 1027-1036.

Lynch, M. and T. J. Crease. 1990. The analysis of population survey data on DNA sequence variation. Molecular Biology and Evolution. 7: 377-394.

Mantelatto, F. L., R. Robles and D. L. Felder. 2007. Molecular phylogeny of the western Atlantic species of the genus Portunus ( Crustacea, Brachyura, Portunidae ). Zoological Journal of the Linnean Society. 150: 211-220.

Moritz, C. 1994. Defining ‘Evolution Significant Units’ for conservation. Tree. 9(10): 373-375.

Mortiz, C. 1995. Uses of molecular phylogenies for conservation. Philosophical Transactions: Biological Sciences. 349(1327): 113-118.

Nelson, G. and N. Platnick. 1984. Biogeography. Carolina Biology Reachers. 119: 1-20.

Nei, M. and F. Tajima. 1983. Maximum likelihood estimation of the number of nucleotide substitution from restriction site data. Genetics. 105: 207-217.

Nei, M. 1987. Molecular evolutionary genetics. Columbia University Press. New York. 512 pages.

Nei, M. and J. C. Miller. 1990. A simple method for estimating average number of nucleotide substitutions within and between populations from restriction data. Genetics. 125: 873-879.

Nei, M. and S. Kumar. 2000. Molecular evolution and phylogenetics. Oxford University Press. New York. 333 pages.

Ng, N. K., J. Y. Guo and P. K. L. Ng. 1999. Genetic affinities of Eriocheir leptognathus and E. formosa with description of a new genus ( Brachyura: Grapsidae: Varuninae ). Journal of Crustacean Biology. 19: 154-170.

Ogoh, K. and Y. Ohmiya. 2005. Biogeoraphy of luminous marine ostracod driven irreversibly by the Japan current. Molecular Biology and Evolution. 22(7): 1543-1545.

Okimoto, R., J. L. Macfarlane, D. O. Clay and D. R. Wolstenholm. 1992. The mitochondrial genomes of two nematodes Caenorhabditis elegans and Ascaris suum. Genetics. 130: 471-498.

Paetkau, D. 1999. Using genetics to identify intraspecific conservation unit: a critique of current methods. Conservation Biology. 13(6): 1507-1509.

Page, R. D. M. and E. C. Holmes. 1998. Molecular evolution: A phylogenetic approach. United Kingdom University Press. Cambridge. 352 pages.

Peng, W. H. 1986. Preliminary study on the problem of variation of Eriocheir sinensis in Zhujiang River valley. Information of Fisheries Science and Technology. 2: 19-22.

Pierce J. C., G. Tan and P. M. Gaffney. 2000. Delaware Bay and Chesapeake Bay populations of the horseshoe crab Limulus polyphemus are genetically distinct. Estuaries. 23(5): 690-698.

Remerie, T., T. Bourgois, D. Peelaers, A. Vierstraete, J. Vanfleteren and A. Vanreusel. 2006. Phylogeographic patterns of the mysid Mesopodopsis slabberi ( Crustacea, Mysida ) in Western Europe: evidence for high molecular diversity and cryptic speciation. Marine Biology. 149: 465-481.

Robbins, R. S., M. Sakari, S. N. Baluchi and P. F. Clark. 2006. The occurrence of Eriocheir sinensis H. Milne Edwards, 1853 ( Crustacea: Brachyura: Varunidae ) from the Caspian Sea region, Iran. Aquatic Invasions. 1(1): 32-34.

Roman, J. and S. R. Palumbi. 2004. A global invader at home: populations structure of the green crab, Carcinus maenas, in Europe. Molecular Ecology. 13: 2891-2898.

Rozas, J. and R. Rozas. 1999. DnaSP version 3.0: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics. 15: 174-175.

Saitou, N. and M. Nei. 1987. The neighbor–joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 4: 406-425.

Sakai, T. 1983. Description of new genera and species of Japanese crab, together with systematically and biogeographically interesting species. Researches on Crustacea. 12: 1-44.

Schmidt, D. and J. Pool. 2002. The effect of population history on the distribution of the Tajima’s D statistic. Cornell University Press. New York.

Schubart, C. D., R. Diesel and S. B.Hedges. 1998. Rapid evolution to terrestrial life in Jamaican crabs. Nature. 393: 363-365.

Schubart, C. D., J. E. Neigel and D. L. Felder. 2000. Use of the mitochondrial 16S rRNA gene for phylogenetic and population studies of Crusracea. Crustacean Issues. 12: 817-830.

Shih, H. T., P. K. L. Ng and H. W. Chang. 2004. Systematics of the genus Geothelphusa ( Crustacea, Decapoda, Brachyura, Potamidae ) from southern Taiwan: A molecular appraisal. Zoological Studies. 43(3): 561-570.

Shih, H. T., G. X. Chen and L. M. Wang. 2005. A new species of freshwater crab ( Decapoda: Brachyura: Potamidae ) from Dongyin Island, Matsu, Taiwan, defined by morphological and molecular characters, with notes on its biogeography. Journal of Natural History. 39(31): 2901-2911.

Shih, H. T., H. C. Hung, C. D. Schubart, C. A. Chen and H. W. Chang. 2006. Intraspecific genetic diversity of the endemic freshwater crab Candidiopotamon rathbunae ( Decapoda, Brachyura, Potamidae ) reflects five million years of the geological history of Taiwan. Journal of Biogeography. 33: 980-989.

Shih, H. T., P. K. L. Ng, C. D. Schubart and H. W. Chang. 2007. Phylogeny and phylogeography of the genus Geothelphusa ( Crustavea: Decapoda, Brachyura, Potamidae ) in southwestern Taiwan based on two mitochondrial genes. Zoological Science. 24: 57-66.

Shy, J. Y. and H. P. Yu. 1992. Complete larval development of the mitten crab Eriocheir rectus Stimpson, 1858 ( Decapoda, Brachyura, Grapsidae ) reared in the laboratory. Crustaceana. 63: 277-290.

Simonsen, K. L., G. A. Churchill and C. F. Aquadro. 1995. Properities of statistical test of neutrality for DNA polymorphism data. Genetics. 141: 413-429.

Sun, H. Y., K. Y. Zhou and D. X. Somg. 2005. Mitochondrial genome of the Chinese mitten crab Eriocheir japonica sinenesis ( Brachyura: Thoracotremata: Grapsoidea ) reveals a novel gene order and two target regions of gene rearrangements. Gene. 349: 207-217.

Tajima, F. 1989. Statistical methods to test for nucleotide mutation hypothesis by DNA polymorphism. Genetics. 123: 585-595.

Tang, B. P., K. Y. Zhou, D. X. Song, G. A. Yang and A. Y. Dai. 2003. Molecular systematics of the Asian mitten crabs, genus Eriocheir ( Crustacea: Brachyura ). Molecular Phylogenetics and Evolution. 29(2): 309-316.

Wallace, A. R. 1998. Island life. Cosimo Classics. New York. 522 pages.

Wenink, P. W., A. J. Baker and M. G. Taianus. 1993. Hypervariable control region sequence reveal global population structuring in a long-distance migrant shorebirds, the Dunlin ( Calidris alpina ). Proceedings of National Academic Science. USA.

Wright, S. 1931. Evolution in Mendelian populations. Genetics. 16: 97-159.

Wright, S. 1951. The genetical structure of populations. Anne Eugenics. 15: 323-354.

Wright, S. 1978. Evolution and the genetics of populations. Vol.4. Variability within and among natural population. University of Chicago press, Chicago. 590 pages.

Yamasaki, I., G. Yoshizaki, M. Yokota, C. A. Strüssmann and S. Watanabe. 2006. Mitochondrial DNA variation and population structure of the Japanese mitten crab Eriocheir japonica. Fisheries Science. 72: 299-309.

Yeo, D. C. J., H. T. Shih, R. Meier and P. K. L. Ng. 2007. Phylogeny and biogeography of the freshwater crab genus Johora ( Crustacea: Brachyura: Potamidae ) from the Malay Peninsula, and the origins of its insular fanua. Zoologica Scripta. 36(3): 255-269.

Zaccara S., F. Stefani and G. Crosa. 2005. Diversity of mitochondrial DNA of the endangered white-clawed crayfish ( Austropotamobius italicus ) in the Po River catchment. Freshwater Biology. 50: 1262-1272.

Zhao, J. H., T. J. Lam and J. Y. Guo. 1997. Acute toxicity of ammonia to the early stage-larvae and juveniles of Eriocheir sinensis H. Milne-Edwards, 1853 ( Decapoda: Grapsidae ) reared in the laboratory. Aquaculture Research. 28: 517-525.

Zhao, N., N. Du, X. Bao and L. Zhang. 1988. Artificial breeding, propagation and culture of Chinese mitten crab. Anhui Science and Technology Press. 10: 134-136.