本研究是以一新穎的組織工程技術來發展修復關節軟骨的方法。材料的選擇方面，鈣磷酸鹽類的生醫陶瓷由於富含鈣和磷離子，和骨組織的成分相近，所以大部分的研究多將鈣磷酸鹽類當作培養硬骨細胞的支架，不過近年來的研究發現其培養軟骨細胞亦有不錯成效。

經評估後本論文使用β-三鈣磷酸鹽（β-tricalcium phosphate，β-TCP）來製作高緻密性的支架，其孔隙度僅有3vol％且孔徑趨近於0；聚磷酸鈣（calcium polyphosphate，CPP）可製作三種不同表面相態（CPP1為β相淬火，CPP2為晶相，CPP3為γ相）的多孔性支架，其孔隙度均為35∼40vol％左右，孔徑以CPP1為最大（達150um），機械強度以CPP1和CPP2較佳，而降解時間以CPP1較合適。

在探討不同的培養方式對培養軟骨細胞的影響時，發現到比起静置培養，懸浮培養除了稍微降低了細胞貼附的能力之外，其他不管是對細胞的增生或是分泌細胞間質的能力都有顯著的提升。在探討不同性質的支架對培養軟骨細胞的影響時，發現到比起CPP2和CPP3，CPP1不管是對細胞的貼附和增生或是分泌細胞間質的能力都比較好。綜合材料和生物性質測試的結果，β相淬火（CPP1）的陶瓷片為一合適的關節軟骨組織工程支架。

TCP陶瓷片為一相當緻密的分隔層材料，此分隔層材料成功的與軟骨區與硬骨區形成了一複合式的支架結構。在探討複合式支架對培養軟骨細胞的影響時，發現到比起無分隔層的支架，有分隔層的支架對細胞貼附和增生的能力都比較好，但分泌細胞間質的能力稍微降低。

In the thesis, the methods of repairing articular cartilage is developed by a novel tissue engineering technique. Calcium phosphates contain ions of calcium and phosphate whick resemble the compostion of native bone. Thus, they are frequently used as material for scaffold on the cultivation of osteocytes. In recent studies, calcium phosphates also showed good results on the cultivation of chondrocytes.

After estimation, we fabricated a dense scaffold by β-tricalcium phosphate（β-TCP）. The porosity is only 3vol%, and the pore size is almost zero. Calcium polyphosphate（CPP） can be used to manufacture a porous scaffold with three different surface phases（CPP1 is β phase with quenching，CPP2 is β phase，CPP3 is γ phase）, and the porosity is around 35~40vol%. CPP1 has the largest pore size（150um）. CPP1 and CPP2 has better mechanical properties. CPP1 has more suitable degradation time.

On the cultivation of chondrocytes by different methods, we found that suspension culture can improve cell growth and cell matrix secretion, although it reduced cell adhesion slightly. As to the effect of the three different surface phases on the cultivation of chondrocytes, we found that CPP1 is the best in cell adhesion, growth ,and cell matrix secretion. In conclusion, the ceramics of β phase with quenching（CPP1） is fit to be a proper tissue engineering scaffold for articular cartilage.

On the cultivation of chondrocytes by complex scaffold, we found that complex scaffold can improve cell adhesion and growth, but it reduce cell matrix secretion slightly.

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