本研究旨在研究顆粒物質與呼吸道微生物族群關係，採用NIST國家標準暨技術研究院所分析出各成份指標樣品做為空氣汙染物樣品，分別將一種人為汙染物(SRM 1633c-Trace Elements in Coal Fly Ash)及兩種自然汙染物(SRM 2709-San Joaquin Soil、SRM 2711-Montana II Soil)施予S.aureus和S.epidermidis上，觀測不同濃度汙染物對細菌的影響，並探討細菌間的消長關係。
受汙染物1633c的影響下，當濃度由900 g/m3降至900 μg/m3，對S.aureus的增殖增量百分比從282.5%逐漸降至38.2%；而S.epidermidis在900 g/m3濃度下則衰退75.1%`,但當1633c濃度降低為900 μg/m3，S.epidermidis與控制組相比反而增殖量增加了22.4%。
受汙染物2709的影響下，當濃度由900 g/m3降至900 μg/m3，對S.aureus的增殖增量百分比從2136.8%逐漸降至33.2%；當2709濃度為900 g/m3時，S.epidermidis的生長衰退97.1%，但當濃度為900 mg/m3的影響下，S.epidermidis與控制組相比反而增殖量增加了40.4%。汙染物2711對兩種菌種的影響與2709相似，當濃度由900 g/m3降至900 μg/m3，對S.aureus的增殖增量百分比從2011.1%逐漸降至-13.6%；而當2711濃度為900 g/m3時，S.epidermidis的生長衰退98.0%，但當2711濃度為900 mg/m3的影響下，S.epidermidis增殖增量增加了66.5%。顯示在同為自然汙染物的影響下，兩種菌種對汙染物的反應較為接近。
由本研究結果可觀察出受相同汙染物影響之致病菌(S.aureus)與非致病菌(S.epidermidis)的增殖呈現負相關，證實汙染物確實會對鼻腔菌落內不同菌種的消長關係產生影響，未來希望能利用微流道設計產出不同汙染物濃度，且將汙染物轉化為氣溶膠態，噴灑於細菌上，藉以模擬汙染物實際掉落在鼻腔黏膜菌落上的情況，並觀察不同菌種間的交互作用及生長模式。

Air pollution, which can be characterized with a high concentration of Particular Matter (PM), is a major concern in areas of dense human activities because it may cause many respiratory disorders, cardiovascular diseases and even cancers.
The purpose of this research is to study the close relationship between environmental Particular Matter (PM) and microbial population in human nasal cavity. Three sources of PM were studied (SRM 1633c-Trace Elements in Coal Fly Ash; SRM 2709-San Joaquin Soil; SRM 2711-Montana II Soil), on two bacterial strains found in human microbiota: Staphylococcus aureus and Staphylococcus epidermis.
Results have shown that different PM concentrations have a strong impact on S.aureus and S.epidermis growth or decline.
Under the effect of 1633c pollutant, as the concentration of S.aureus reduces from 900 g/m3 to 900 μg/m3, the percentage of proliferation increasement of S.aureus gradually declines from 282.5% to 38.2%. Moreover, those of S.epidermidis declines to 75.1%. And yet, as the concentration of 1633c reduces to 900 μg/m3 , the percentage of proliferation increasement of S.epidermidis compares with control increases 22.4% instead.
Under the effect of 2709 pollutant, as the concentration of S.aureus reduces from 900 g/m3 to 900 μg/m3 , the percentage of proliferation increasement of S.aureus gradually declines from 2136.8% to 33.2%. When the concentration of 2709 is 900 g/m3 , the growth of S.epidermidis declines 97.1% but when the percentage of proliferation increasement of it, under the effect of 900 mg/m3, compares with control increases 40.4%. The effect of 2711 pollutant is similar to 2709 pollutant. As the concentration reduces from 900 g/m3 to 900 μg/m3, the percentage of proliferation increasement of S.aureus gradually declines from 2011.1% to -13.6%. In addition to, as the concentration of 2711 is 900 g/m3, the growth of S.epidermidis declines 98.0%; however, under the effect of 900 mg/m3 2711, the percentage of proliferation increasement of S.epidermidis increases 66.5%. The results shows under the effect of same natural pollutants, the reaction of two bacteria to pollutants are much similar.
In conclusion, negative correlations were observed between S.aureus and S.epidermidis proliferation increasement under the different pollutant concentrations tested. PM concentrations may strongly affect nasal microbiota. Future research will be focused on spraying these pollutants in air and study their deposition and growth on nasal cavity.

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