生醫微流體晶片應用於生殖系統方面已經越來越普及。以往用以分選的機制大致可以分為：1.化學梯度吸引 2.熱吸引 3.穩定層流分選 4.逆向流場分選。但這些分選方法中，除了穩定層流外，都會對精子本身活動力有一定的削減。而穩定層流的方法又無法有效地分別出精子的活動力。因此，本研究提出了一個漸擴流場的機制，隨著流道的逐漸增擴，流速將相對的減慢。在流場中的精子感受到流場會對其做抵抗。同時隨著流場的減弱而依照本身運動能力停留在不同區塊，得到分選的效果。在分選漸擴區域，開設一個側枝，準備將分選後的精子取出。由於側枝在分選時做了氣密處理，與入口並無壓力差，因此精子在經過時並不會有流場將之帶入，而是僅依靠擴散的方式進入。側枝流道可以成功的取出高效精子同時不影響分選。分選結束後，將側枝的氣密解除，使用滴管將高效精子取出。如此一來，操作簡易，移除了多數實驗所需要的幫浦，在臨床上也是一大優勢。經過分選的精子，數量約2000至15000隻，存活率達到77%，證實晶片確實可將死亡精子沖洗掉，篩檢出存活精子。活動力方面，Motile sperm由38%提升至70%，Progressive Sperm則是由11%提升至33%，可見經過精片作用，高效能精子成功被取出。最後將分選後的精子與本實驗室的研究” 建構三維結構之微流體裝置應用於鼠卵細胞培養與透明帶去除”所剝殼的老鼠卵子做結合後，與使用傳統方式剝殼並配上未分選精子後做比較，受孕潛力較傳統方式的40%提升到60%。另外，本晶片使用的是PMMA壓克力。配合熱壓模的製程方法，可以以便宜又快速的方式量產。流道入口、出口端接有文字做標示，協助使用者操作，避免人為疏失造成晶片失效。經過封裝後，可將PMMA塑膠晶片做成一可以快速量產的商業產品，應用於人工試管嬰兒。

This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector without affecting the sorting process because the chamber is blocked while sorting. At the end of sorting, the collateral chamber is open and then using just pipette to derive high quality sperms instead of pump. Controllable numbers, ranging from 2000 to 10000, of sperms can be collected with a motility (>70μm) ratio higher than 70%, suitable for in-vitro fertilization (IVF). Hot embossing lithography process is presented. Microchannels are replicated on a Polymethylmetacrylate (PMMA) stamp with thickness of 1 mm. With low cost and high replication precision, we can make it a clinical chip.

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