使用傳統方法進行果蠅嗅覺實驗研究已經解開巨觀上神經網絡之謎，然而受限於實驗方法及輔助工具的不足，勢必要通過更複雜的實驗設計才能一窺微觀尺度的細部神經網絡連結，因此為了有效化簡實驗設計並加速研究基因篩選，本研究提出了一項可結合雷射進行ChR2實驗，應用於研究單隻果蠅於二維空間下的嗅覺行為趨勢即時觀測系統。透過特殊設計的果蠅盒，我們不需剪去果蠅翅膀即可限制其於近乎二維方向上移動，同時我們利用機器視覺的方式擷取果蠅影像，在此應用於機器視覺照明的光源亦不影響ChR2實驗的進行，由於特殊的果蠅治具及相關硬體設置，經由簡單的影像前處理及果蠅辨認步驟，即可得到穩定的果蠅位置資訊，藉由位置資訊進行基本的行為參數定義，最後透過程式執行程序、時序的安排達成即時觀測系統的需求。除了即時觀測模組外，我們亦於程式中加入離線分析模組可進行實驗影片錄製以及果蠅趨勢圖繪製。期望透過我們的系統能夠幫助建立新型態的果蠅行為趨勢研究，並有效率加速相關實驗及研究。

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