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研究生: 黃詩梅
NG SHI MEI
論文名稱: 孔雀薑屬(薑科)植物系統分類研究
A Systematic study on the Genus Kaempferia (Zingiberaceae)
指導教授: 李家維
Li, Chia-Wei
口試委員: 胡維新
Hu, Wei-Hsin
王澤宏
Wang, Liang-Jong
學位類別: 碩士
Master
系所名稱: 生命科學暨醫學院 - 分子與細胞生物研究所
Institute of Molecular and Cellular Biology
論文出版年: 2012
畢業學年度: 100
語文別: 中文
論文頁數: 126
中文關鍵詞: 薑科孔雀薑分類學
外文關鍵詞: Zingiberaceae, Kaempferia, taxonomic
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  • 薑科(Zingiberaceae)孔雀薑屬(Kaempferia)植物分布於印度、中國(雲南)及東南亞等地,以泰國為主要分布區域。根據統計,目前約有30個物種被命名,當中並未包含於2008年至2011年間所發表的8個新物種,尚有約20個物種尚未被命名及發表。Kaempferia屬內有詳盡形態描述之記錄的僅有25個物種。本次研究針對Kaempferia屬,分為兩個部分:第一部分為形態特徵之分類研究,將12個已知,7個未知物種及1個栽培種作了詳細之形態特徵描述。同時也將當中9個已知,4個未知物種及1個栽培種進行花部構造之解剖。最後依據各個物種之形態特徵建立分類檢索表。第二部分則是分子譜系系統分類學研究,以2種葉綠體分子標記:matK局部序列及psbA-trnH intergenic spacer之局部序列進行PCR擴增並定序。再進行最大簡約法、鄰近相接法及最大似然法做譜系關係之分析。最後結合兩部分之研究成果,以期建立Kaempferia屬內之親緣關係與了解演化歷程。結果顯示兩種葉綠體標記分析結果雖有些許差異,但大部分所得之分類群仍相似,並與形態特徵呈一致性之結果。具有由葉鞘抽長之倒鐘狀花序的K. elegans與K. parviflora互為姊妹群;在形態特徵分類上較難辨別之K. marginata、K. galanga與K. laotica在分子譜系中也呈未解,序列上出現多處變異。形態本身變異最大之K. elegans在DNA序列上也出現不少差異。特徵形態上無變異之K. roscoeana在psbA-trnH之局部序列PCR擴增時呈現兩種序列變異,一為長794bp之序列,另一個DNA序列長度只有383bp,在整段序列的中間部分完全缺失。


    The genus Kaempferia L. (Zingiberaceae) distributes in India, China (Yunnan), and South East Asia. Thailand has the highest species diversity. There were about 30 named species, but not included eight new species published between 2008 and 2011. However, about 20 species were unnamed and unpublished. The study includes two parts, classification based on morphological characters and molecular phylogenic analysis. We investigated morphology of 12 identified, 7 unidentified and one cultivated species, and floral anatomy of 9 identified, 4 unidentified species and one cultivated species, to establish a more complete taxonomy. On the other hand, we use two chloroplast DNA markers, psbA – trnH intergenic spacer and matK partial sequence, to make phylogenic analyses.The results showed that two different chloroplast DNA markers made similar pattern and are consistened with the taxonomy based on morphology. K.elegans and K. parviflora had the cyathus inflorescence elongating from leaf-sheath were classified as sister group. The relationships between K.marginata, K. galanga and K. laotica were still unclear. K. elegans had larger variation in nucleotide sequence and morphology. Although had no variation on morphology, K. roscoeana had two sequential variations on psbA-trnH.

    A. Abstract 2 B. 中文摘要 3 壹、 前言 6 貳、 形態特徵分類研究 8 參、 分子譜系系統分類學研究 19 一、 系統分類學研究背景 19 1. Zingiberaceae(薑科植物) 19 2. Kaempferia(孔雀薑屬) 27 二、 分子譜系分析 (Molecular Phylogenetic Analysis) & DNA分子標記 34 肆、 材料與方法 (Materials and Method) 36 伍、 結果 (Results) 41 1. 形態特徵分類研究 41 1. Kaempferia之形態特徵 41 2. Kaempferia之分類檢所表 (Taxonomic key to the Kaempferia species) 42 I. Kaempferia angustifolia 46 II. Kaempferia elegans 48 III. Kaempferia galanga. 50 IV. Kaempferia gilbertii 52 V. Kaempferia laotica 54 VI. Kaempferia marginata 56 VII. Kaempferia minuta 58 VIII. Kaempferia parvifolia 60 IX. Kaempferia pulchra 62 XI. Kaempferia roscoeana 64 XII. Kaempferia rotunda. 66 XIII. Kaempferia siamensis 68 XIV. Kaempferia pulchra Alva 70 XV. Kaempferia sp. Chantaburi K040017 72 XVI. Kaempferia sp. Lop Buri 74 XVII. Kaempferia sp. Mukdahan 76 XVIII. Kaempferia sp. Prachuap 78 XIX. Kaempferia sp. K040073 80 XX. Kaempferia sp. K040638 82 XXI. Kaempferia sp. K040814 84 3. 花柱之形態特徵 85 二、 分子譜系系統分類學研究 92 1. psbA-trnH intergenic spacer之局部序列矩陣資料分析 93 I. 最大簡約法分析結果 93 II. 鄰近相接法分析結果 94 III. 最大似然性分析結果 95 2. 葉綠體matK基因局部序列矩陣資料分析 96 I. 最大簡約法分析結果 96 II. 鄰近相接法分析結果 97 III. 最大似然性分析結果 98 陸、 討論 (Discussions ) 99 一、 形態特徵分類研究 99 1. Kaempferia屬內之分類群劃分 99 2. K. elegans之形態變異 100 3. K. marginata、K. galanga與K. laotica之形態分類問題 104 4. 傳統形態分類學研究之困難 104 二、 分子譜系系統分類學研究 105 1. 葉綠體matK基因與psbA-trnH intergenic spacer區段 105 2. Kaempferia屬內之分類群劃分 105 3. K. elegans之形態變異 106 4. K. marginata、K. galanga與K. laotica之形態分類問題 107 5. K. roscoeana之序列變異 107 6. 傳統形態分類學與分子譜系研究之結合 108 陸、 後續研究方向 (Future works) 109 柒、 參考文獻 (Reference) 110 捌、 附錄文件 114

    吳德鄰 (1994). 薑科植物地理. 熱帶亞熱帶植物學報 2 (2):1 – 14.

    Baker, J.G. (1890-1982). Scitamineae. – In Hooker, J.D., Flora of British India 6, London, pp. 198-264

    Branney, T.(2002). More than a hint of ginger. The Garden 127 (9): 718-723.

    Burtt, B. L. & Olatunji, O. A.(1972). The limits of the tribe Zingibereae. Not. R. B. G. Edinb., 31 (2): 167-169.

    Felsenstein, J. (2004). Inferring phylogeny. Sinauer Associates, Inc., USA. pp. 664.

    Gagnepain, F. (1908). Zingibéracées. In: Lecomte, M. H. (ed.), Flora Génerale de L’ Indochine. 6: 45-54.

    Holttum, R.E. (1950). The Zingiberaceae of the Malay Peninsula. Gard. Bull. Singapore 13: 1-283.

    Jenjittikul, T. & Larsen, K.(2000). Kaempferia candida Wall. (Zingiberaceae), a new record for Thailand. Thai For. Bull. 28: 45-49.

    Kam, Y. K. (1980). Taxonomic studies in the genus Kaempferia (Zingiberaceae). Notes R. Bot. Gard. Edinb. 38(1): 1-12.

    Kirchoff, B. K. (1997). Infloresence and flower development in the Hedychieae (Zingiberaceae): Hedychium. Can. J. Bot. 75: 581-594.

    Kirchoff, B. K. (1998). Infloresence and flower development in the Hedychieae (Zingiberaceae): Scaphochlamys kunstleri (Baker) Holttum. Int. J. Plant Sci., 159(2): 261-274

    Koonterm, S. (2008). Kaempferia chayanii (Zingiberaceae), a new species from Southern Laos. Folia malaysiana, 9: 1-6.

    Kress, W. J. (1990). The phylogeny and classification of the Zingiberales. Ann., 77: 698-721.

    Kress, W. J. (1995). Phylogeny of the Zingiberavae; morphology and molecules. In P. Rudall, P. J. Cribb, D. F. Cutler, and C. J. Humphries [eds.], Monocotyledons: systematics and evolution, pp. 443-460. Royal Botanic Gardens, Kew, UK.

    Kress, W. J., Prince, L.M. & Williams, K.J. (2002). The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. Am. J. Bot. 89: 1682-1696.

    Kress, W. J., Liu, A. Z., Newman, M. & Li, Q. J.(2005a). The molecular phylogeny of Alpinia (Zingiberaceae): a complex and polyphyletic genus of gingers. Am. J. Bot. 92: 167-178.

    Kress, W.J., Wurdack, K.J., Zimmer, E.A., Weigt, L.A., et al. (2005b). Use of DNA barcodes to identify flowering plants. Proc. Natl. Acad. Sci. U. S. A. 102: 8369-8374.

    Larsen, K. (1962). Studies in Zingiberaceae of the Malay Penisula. – Gard. Bull. Singapore 13: 1-249.

    Larsen, K., Lock, J. M., Maas, H., & Maas, P. J. M.(1998). Zingiberaceae. In. Kubitzki, K. ed. The families and genera of vascular plants: IV Flowering Plants. Monocotyledons: Alismatanae and Commelinanae (except Gramineae). Berlin: Springer. pp. 474-495.

    Linnaeus, C. (1753). Monandria. In Species Plantarum-A facsimile of the first edition. London: Bernard Quaritch Ltd.

    Loesener, T. (1930). Zingiberaceae. pp. 541-640 in Naturliche Pflanzenfamilien, 2nd, 15a., eds A. Engler and K. Prantl, Leipzig: W. Engelmann

    Ngamriabsakul, C., Newman, M. F. & Cronk, Q. C. B. (2003). The phylogeny of tribe Zingibereae (Zingiberaceae) based on ITS (nrDNA) and trnL-F (cpDNA) sequences. Edinburgh J. Bot. 60: 483-507

    Hebert P. D. N., Cywinska, A., Ball, S.L., & Waard J.R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, Series B 270: 313-321.
    Picheansoonthon, P. & Koonterm, S. (2008). Notes on the genus Kaempferia L. (Zingiberaceae) in Thailand. J. Thai Trad. Alt. Med. 6: 73-93.

    Picheansoonthon, P. & Koonterm, S. (2008). A New Species of Kaempferia (Zingiberaceae) from Southern Laos. Taiwania 53(4): 406-409.

    Picheansoonthon, P. (2009). Kaempferia sawanensis (Zingiberaceae), A New Species from Southern Laos. Acta Bot. Yunnan. 31(6): 509-512

    Picheansoonthon, P. & Koonterm, S. (2009a). A New Species of Kaempferia L. (Zingiberaceae) from Northeastern Thailand. Taiwania 54(1): 52-56.

    Picheansoonthon, P. & Koonterm, S. (2009b). Two new Kaempferia L. (Zingiberaceae) from Southern Laos. Taiwania 54(3): 219-224.

    Picheansoonthon, P. (2010). Kaempferia lopburiensis (Zingiberaceae), a New Species from Central Thailand. J. Jap. Bot. 85(3): 148-152

    Picheansoonthon, P. (2011). Two new Kaempferia (Zingiberaceae) from Thailand. J. Jap. Bot. 86(1): 1-7

    Ridley, H.N. (1924). Flora of the Malay Peninsula 4, London, pp. 1-458.

    Saensouk, S. & Jenjittikul, T. (2001). Kaempferia grandifolia sp. nov. (Zingiberaceae) a new species from Thailand. Nord. J. Bot. 21: 139-142.

    Saitou, N. & Nei, M. (1987). The neighbor-joining method: a new method for reconstructed phylogenetic tree. Mol. Biol. Evol. 4: 406-425.

    Schumann, K. (1904). Zingiberaceae. In: Engler, A. ed. Pflanzenreich IV. 46. Leipzig: W. Engelmann, pp. 64-88

    Searle, R.J. (1999). A new combination and new synonymy in Kaempferia (Zingiberaceae: Hedychieae). Telopea 8: 375-376.

    Sirirugsa, P. (1989). The genus Kaempferia (Zingiberaceae) in Thailand. Nord J Bot. 9: 257-260.

    Sirirugsa, P. (1991). Taxonomy of the genus Kaempferia (Zingiberaceae) in Thailand. Thai For. Bull. 19: 1-15.

    Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 28: 2731-2739.

    Techaprasan, J., Ngamriabsakul, C., Klinbunga, S., Chusacultanachai, S. et al.(2006). Genetic variation and species identification of Thai Boesenbergia (Zingiberaceae) analyzed by chloroplast DNA polymorphism. J. Biochem. Mol. Biol. 39: 361-370.

    Techaprasan, J., Klinbunga, S., Ngamriabsakul, C. & Jenjittikul, T.(2010). Genetic variation of Kaempferia (Zingiberaceae) in Thailand based on chloroplast DNA (psbA-trnH and petA-psbJ) sequences. Genet. Mol. Res. 9: 1957-1973.

    The Plant List (2010). Version 1. Published on the Internet; http://www.theplantlist.org/ (accessed 1st January).

    Thompson, J. D., Higgins, D. G. & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nuc. Ac. Res. 22, 4673-4680.

    Valeton, Th. (1918). New note on the Zingiberaceae of Java and Malaya. Bull. Jard. Buitenzorg. Serie 2. 27: 1-87

    Williams, K., Kress, W.J. and Manos, P.S. (2004). The phylogeny, evolution, and classification of the genus Globba and tribe Globbeae (Zingiberaceae): appendages do matter. American J. Bot. 91: 100-114.

    Wu, T.L. & Larsen, K. (2000). Family Zingiberaceae. In Z.-G. Wu and P. H. Raven, Flora of China 24: 322–377. Science Press, Beijing, China, and Missouri Botanical Garden Press, St. Louis, Missouri, USA.

    Xia, Y. M., Kress, W. J. & Prince, L. M. (2004). Phylogenetic analysis of Amomum (Alpinioideae: Zingiberaceae) using ITS and matK DNA sequence data. Syst. Bot. 29 (2): 334-344.

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