目前蛋白質晶片在傳統酵素連結免疫吸附分析法(Enzyme Linked-Immuno-Sorbent Assay,ELISA)檢測上所遇到的問題，除了蛋白質抗體固定於晶片上的效率外，因為抗體在同一種自組裝單分子層(Self-assembled monolayers,SAMs)的表面上固定(immobilize)，因為緊密堆積而有立體空間障礙問題和方向性問題，導致和檢體抗原做反應時無法達到最有效的結合造成檢體的浪費。因此我們提出了利用不同功能、不同高度的矽烷類混合式自組裝單分子膜，分別是(3-Aminopropyl)trimethoxysilane(APTS)及Ethyltrimethoxysilane(C2)兩種官能基相似的化學分子，將抗體Goat anti-rabbit IgG豎立於晶片表面上的方法，藉由控制兩種SAMs分子的分佈比例與鍵結效率就可以提高抗體的自由度，而達到立體空間障礙的解決，使末端的抗原接合端(antigen binding site)得以大部分裸露出來，讓後續的抗原Rabbit anti-mouse IgG在和抗體接合時效率提高，以減少數量上的浪費。
由實驗結果得知，抗原對抗體的接合效率在APTS/C2體積混合比為1:10時可得到較佳(Optimal)的值，大約為0.3；相較於傳統單純APTS的表面效率為0.04；抗原對抗體的接合效率大致上提高了7.5倍左右，成功的達到本實驗設計的目的。

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