本研究以治療MCF-7/ADR抗藥性癌細胞為目的，製備出一具標靶功能之溫度敏感產氣式微脂體藥物控釋系統。為了提高細胞對該載體的吞噬效率，並讓系統能藉由胞吞作用直接進入細胞，我們針對MCF-7/ADR細胞膜上的nucleolin蛋白為標定對象，在微脂體外接能標定nucleolin蛋白的適體(aptamer)，同時結合載體內部碳酸氫銨的溫度觸發釋藥機制，輔助藥物在細胞內大量釋放。本研究規劃為三個部分：首先是載體系統型態分析、包覆率和藥物釋放行為觀察，以確認其做為一溫度敏感性藥物載體之可行性；接著，探討有無接枝適體之載體被細胞吞噬之能力，並使用顯微鏡追蹤細胞吞噬載體之路徑，以選擇載體適當的釋藥時間點。最後，觀察接枝適體之載體，在不同環境溫度下，攜帶DOX累積於細胞內的情形，及對癌細胞的毒殺效果。而由實驗結果可證實，此系統能有效躲避抗藥性細胞P-gp的抗藥機制，並藉由環境溫度的變化觸發載體藥物釋放，讓藥物在短時間於胞內累積至致死濃度，達到毒殺MCF-7/ADR抗藥性癌細胞的作用。

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