對蛋白質的胜肽鍵(-CO-NH-)具有水解反應之催化性的酵素統稱為蛋白質水解酵素，其中鹼性絲氨酸蛋白質水解酵素(alkaline serine protease)有特別優越的經濟效益，最具代表性。鹼性絲氨酸蛋白質最大的特點就是在鹼性條件下，其活性仍十分穩定，故在應用方面是以清潔劑工業為主，但是同時也可以用於肉品、皮革加工、乳製品、食品加工方面。本論文在較佳的操作參數下，分別以共價固定法、活化法以及雙官能基固定法，將鹼性蛋白質酵素固定於幾丁聚醣上，其中以雙官能基固定法有最優越的活性表現。
在生化活性表現上，固定化酵素在環境pH值以及溫度的安定性方面都有所提升。在經過20次的重複使用後，雙官能基固定法的固定化酵素仍有62%的殘留活性，而共價固定法以及活化法的固定化酵素則較差，分別是51%和40%。在動力學參數方面，經過固定化之後的酵素其Km值有上升的趨勢，以雙官能基固定法所得到的酵素有最大的Km值；而Vm值則是有下降的趨勢，以共價固定法所得到的固定化酵素有最小之Vm值。此外，Km值與Vm值不會受交聯/活化時間、交聯/活化pH值與固定化時間等固定化參數的影響。反之，會受交聯/活化濃度、固定化pH值以及固定化溫度的影響。

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