生物體中存在著各種生物超分子(supramolecule)，例如nuclear pore complex, postsynaptic density (PSD), brain melanin granule, ribosome,這些由各種巨分子（Macromolecule）所聚合而成的結構，往往在生物體內扮演重要的角色，藉分析這些生物超分子的組成與結構，我們可以瞭解其作用機制。隨著蛋白質體學技術的發展，對於超分子中蛋白質組成的研究，已經有許多方法學發展成熟，然而對其蛋白質空間排列的研究相對缺乏，本實驗發展出一套方法，用以鑑定蛋白質在超分子中所在位置的深淺分層。我們先以一個已知蛋白質組成的系統three-layered protein complex（TPC）來驗證此方法學的可行性後，再以後突觸質密區（postsynaptic density,PSD）為實驗對象，進行對其空間結構的分析。 根據本篇論文所研發之方法對PSD的分析結果，我們可將PSD蛋白質分為四大類，分別為距表面3.5nm內的蛋白質、距表面3.5∼7nm區域的蛋白質、距表面7nm內的蛋白質以及距表面超過7nm的蛋白質，整理以上結果，可描繪出在PSD中蛋白質分層的情形，而除了可用於對PSD的研究之外，本篇論文同時也提供了分析其他超分子空間結構的一個方法學。

劉思□(2006), 研發固相化學交聯方法學以研究豬大腦後突觸質密區組成蛋白質之排列結構 / Studying the Protein Organization of the Postsynaptic Density by a Novel Solid Phase- and Chemical Cross-linking-based Technology, 國立清華大學生命科學研究所博士論文
游珮均(2006), 利用化學交聯法分析後突觸質密區(PSD)之蛋白結構 / Analysis of protein organization of the postsynaptic density by chemical cross-linking method, 國立清華大學分子醫學研究所碩士論文
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