中文摘要
細胞內的鈣離子通透對於間葉細胞分化成軟骨細胞的初期是很重要的，但對於受電流調控活性的鈣離子通道蛋白 (voltage-gated calcium channel) 與間葉細胞(mesenchymal cell) 分化成軟骨細胞 (chondrocyte) 的機制研究，仍然不清楚。目前，我們實驗室發現，T-型鈣離子通道 (Cav3.2) 對於小鼠氣管軟骨正常生成是很重要的。Cav3.2基因缺失小鼠的呼吸道中，形成先天性的氣管狹長，原因是在發育的過程中，氣管軟骨環發育缺失所造成的現象。同時我們利用大量轉殖Cav3.2基因表現到軟骨前驅細胞株 (chondroprogenitor cell, ATDC5 cell) ，發現大量表現Cav3.2基因的軟骨前驅細胞株有促使明顯的分化軟骨形成 (chondrogenesis) 。此增強的軟骨分化現象受到T-型鈣離子通道阻斷劑和calcineurin活性抑制劑給破壞。除此，我們發現參與調控軟骨生成的主要因子Sox9蛋白質，在Cav3.2缺陷小鼠的氣管發育中，表現量比正常小鼠低。更進一步研究發現，Sox9蛋白基因的上游調控區有一個NFAT蛋白結合位置，這個結合位置與鈣離子透過Cav3.2進入細胞調控Sox9蛋白的表現有關。我們的實驗結果顯示，鈣離子經由Cav3.2進入細胞，透過活化calcineurin/NFAT蛋白的訊號傳遞路徑，調控小鼠氣管軟骨生成的過程中Sox9蛋白的表現。

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