中文摘要
第一型人體免疫缺乏病毒(HIV-1)屬於反轉錄病毒的一種，表現反轉錄病毒的三個基本組成蛋白分子：Gag、Pol和Env；Gag上包含四個主要的區域：MA、CA、NC和p6，其中CA會組成病毒的殼層。之前的研究發現CA的蛋白質序列，從MHR起C端末約為整個CA的三分之ㄧ就足夠形成病毒粒子；我們的研究發現緊鄰MHR　C端的序列1715-1758對於病毒的產生有重要的影響，這段序列中的胺基酸Q179、K182、W184突變成Alanine時會嚴重抑制病毒的產生，突變的Gag蛋白分子在細胞內的分佈並沒有改變，病毒無法產生的原因可能是無法形成高分子量的分子；另外我們也發現，在不同的反轉錄病毒中，從MHR到緊鄰MHR C端的序列，可能具有相類似的結構。

英文摘要
HIV-1 belongs to Retroviruses. HIV-1 expresses three basic components of retroviruses: Gag, Pol and Env. Gag protein can be devided into four major domains: MA, CA, NC amd p6. CA constitutes the core structure of a virus particle. In CA, previous research suggested that the region from MHR (major homology region) to the C terminus of CA is enough to produce a virus particle. Here, we showed that the sequence 1715-1758 is important for virus production. Of the ten CA mutants, Q179A, K182A and W184A caused severe defects in directing virus assembly and release. Although these mutants showed a subcellular distribution profile close to that of wild type, they appeared to be impaired to a certain degree in multimerization. Besides, we also found that different retroviruses may have similar structures from MHR to the region adjacent to C-terminus of MHR.

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