在目前癌症治療上，對於某些特定的腫瘤，放射線是常見的治療方式之一。放射線的優點在於可以針對一限制區域的腫瘤進行治療，但治療的過程中還是無法避免照射到正常的組織細胞。當細胞受到放射線的照射後，可能會表現出不同程度和型態的反應，如發炎及免疫反應。目前文獻資料中還沒有放射線與正常細胞的基因表現相關的研究，因此我們首先想了解正常細胞在受到放射線照射後，其免疫相關基因在短時間內的改變。另外我們同時也可以觀察不同型態功能的器官，受到放射線照射後其免疫反應的不同。實驗中我們以C57BL/6J老鼠為實驗對象，分別觀察在放射線的照射後，其八種器官(包括：腦、肺臟、肝臟、腎臟、胰臟、小腸、膀胱和前列腺)在不同時間點上免疫相關基因表現上的改變。另外，我們以注射LPS引發免疫系統反應的C57BL/6J老鼠作為實驗的對照組。從實驗中我們發現，C57BL/6J老鼠在放射線照射後，其腎臟、膀胱和前列腺這三個器官在我們欲觀察的免疫相關基因表現上，和控制組比較起來沒有明顯差異。在腦、肺臟、肝臟、胰臟和小腸等五個器官，分別在TNF-alpha、IL-5、IL-1alpha、INF-gamma、IL-1beta和IL-6的基因表現上都有不同程度改變；其中IL-1beta則是唯一在五種器官均有改變的。根據這樣的結果，我們可以進一步改善在照射治療後，降低老鼠體內引發過度的免疫反應，使細胞可以維持正常的生理功能。

Radiotherapy is a front treatment for many types of cancer. One of its advantages is to target specific tumor site, however under some condition, there are still risks to induce complications in the surrounding normal tissues. The mechanisms of radiation-induced normal tissue damage are complicated and involve different tissue components such as epithelial cell, stromal cells, inflammatory and immune responses. Our previous studies have found induction of pro-inflammatory are involved in early phases of normal tissue responses to radiation. The purpose of this study is to extend our previous observation and compare the short-term induction of pro-inflammatory cytokines in different organs following whole body irradiation.
We used C57BL/6J mice as an animal model and analyzed gene expression of pro-inflammatory cytokine in eight organs (brain, lung, liver, kidney, spleen, intestine, bladder and prostate) at different time following whole body irradiation. LPS was used as a positive control for the expression pattern of cytokine genes in a variety of organs following inflammatory stimulation. Among these organs examined, kidney, bladder and prostate did not show induction of pro-inflammatory cytokine genes following irradiation. The other organs including brain, lung, liver, spleen and intestine showed different pattern of TNF-alpha, IL-1alpha, INF-gamma, IL-1beta and IL-6 gene induction, and increase of IL-1beta expression was found in all of these five organs.
Our studies clearly showed that, in terms of early pro-inflammatory response, different organs respond to radiation damage in a different pattern in their type of cytokine genes, time of expression and intensity of expression. These results provide important information in our understanding radiation-induced early pro-inflammatory responses in different normal tissues.

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