研究生: |
林鴻嘉 Lin, Hung-Chia |
---|---|
論文名稱: |
介電濕潤液珠操控技術開發與生醫檢測應用 Development and medical diagnostics of an EWOD droplet manipulation technology |
指導教授: |
饒達仁
Yao, Da-Jeng |
口試委員: | |
學位類別: |
博士 Doctor |
系所名稱: |
工學院 - 奈米工程與微系統研究所 Institute of NanoEngineering and MicroSystems |
論文出版年: | 2009 |
畢業學年度: | 97 |
語文別: | 中文 |
論文頁數: | 118 |
中文關鍵詞: | 介電濕潤 、液珠操控 、生醫 、數位微流體系統 |
外文關鍵詞: | EWOD, Droplet manipulation, Biomedical, Digital microfluidic system |
相關次數: | 點閱:3 下載:0 |
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本研究係開發一項介電溼潤液珠操控技術,並搭配此項技術開發也自行組配了雙平面式液珠操控實驗平台與共平面式液珠操控實驗平台。本實驗平台已具備了基本的液珠傳輸、混合、分離及成型等四大基本功能,並針對生醫檢體進行了驅動測試。目前本實驗平台可操控的生醫檢體包含了生殖醫學上常用的試管嬰兒胚胎植入培養液(IVF medium)、公豬豬精、去氧核糖核酸(deoxyribonucleic acid,DNA)、線性選殖載體(linearized cloning vector)、酵素(enzyme)以及磷酸鹽(phosphate-buffered saline,PBS)等緩衝溶液。透過共平面式液珠操控實驗平台的建構,可完成一個開放式的數位微流體系統,使用者可以更有彈性的進行液珠的傳輸及混合等反應,若要進行液珠成型的機制只要外加一個疏水上蓋即可完成,另外本晶片皆利用透明的ITO 玻璃來製作,在後續的光學檢測及影像擷取皆非常便利。
為了能將此液珠操控技術實際應用在生醫檢測上,所以本研究所開發之實驗平台必須具備自動化進樣系統的功能,透過此系統的規劃可前置處理生醫檢測上所需不同濃度的檢體與試劑,為了驗證此項功能便透過葡萄糖序列稀釋實驗來完成,透過實驗結果可知利用本實驗平台所處理完成的液珠濃度與利用傳統pipette所調配出來的液珠濃度誤差在5%以下,證實了本實驗平台具備了自動化進樣系統的功能。最後本研究也利用共平面式液珠操控實驗平台完成了核酸接合反應(DNA ligation)的實驗,此外本實驗也探討了不同試劑的混合次序及時間對於DNA cloning的影響。
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