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| 研究生： |
簡弘昌 |
|---|---|
| 論文名稱： |
合成新型含腙類化合物之金奈米粒子作為去氧核醣核酸(DNA)切割材料 Synthesis of Hydrophobic Hydrazone-Integrated Gold Nanoparticles as DNA-Cleaving Materials |
| 指導教授： | 胡紀如 |
| 口試委員: | |
| 學位類別： |
碩士 Master |
| 系所名稱： |
理學院 - 化學系 Department of Chemistry |
| 論文出版年： | 2006 |
| 畢業學年度： | 94 |
| 語文別： | 英文 |
| 論文頁數： | 29 |
| 中文關鍵詞： | 腙類化合物 、金奈米粒子 、去氧核醣核酸(DNA)切割材料 |
| 相關次數： | 點閱：4 下載：0 |
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於本篇論文中，本人合成了兩種新型含有腙類化合物之新型疏水性金奈米粒子。藉由紫外光(波長312-nm)的照射二小時條件下，這些掺雜腙類配位基與均一相腙類的金奈米粒子在1 □M及10 nm的濃度下，於磷酸緩衝溶液中對去氧核醣核酸具有切割效果。以預先製備好的長碳鍊硫醇保護之金奈米粒子，透過配位基交換系統可得到掺雜腙類配位基的有機相金奈米粒子;而利用硼氫化鈉還原金離子並與有機硫醇分子在甲苯溶液中反應生成均一相的腙類奈米粒子。
Two types of new hydrophobic organic gold nanoparticles were synthesized, which contained hydrazone moiety. Upon irradiation with 312-nm UV light, these integrated gold nanoparticle-hydrazone hybrids cleaved DNA at the concentrations as low as 1 □M and 10 nM for hybrids with mixed and homogenous organic ligands, respectively. The mixed hybrids were prepared from pre-formed organothiolated gold nanoparticles through ligand exchange; the homogeneous hybrids were prepared by reduction of HAuCl4 with NaBH4 in the presence of functional thiolated molecules in toluene.
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