本文計畫開發一細胞檢測晶片，將傳統流式細胞儀的一維線性判讀改為二維平面檢測，如此可大幅提升檢測效率。二維平面檢測係利用聚苯乙烯球的自組裝特性來預測細胞也能有相同的自組裝排列，利用水流作用力帶動細胞的原理使細胞產生緊密整齊的單層排列，如此可輕易地在螢光顯微鏡下觀察細胞的二維陣列，以利判讀癌細胞，且因是單層，不會導致訊號遮蔽的問題。
然而在癌症的檢測上需要立即性，若是以液體蒸發方式讓細胞產生自組裝則需時太久，本研究利用Poly(acrylic acid) partial sodium salt的吸水特性與Polyethylene terephthalate 膜的接合開發一平台，使細胞懸浮液中的液體能快速地在此平台上被吸收，使細胞產生自組裝。本文利用此平台作幾項測試，第一項為細胞產生自組裝的測試，本研究成功地找到一產生自組裝的細胞濃度，能夠使細胞緊密的單層排列，似聚苯乙烯球自組裝排列時的六方最密堆積現象；第二項為癌細胞比例判讀的測試，讓細胞染上不同螢光，作為癌細胞與正常細胞的區別，待細胞懸浮液在平台上產生自組裝排列後，由顯微鏡觀測計數癌細胞的比例是否與理想值相符，本實驗數值都與理想值十分接近，在95%信心水準之下誤差值為5.58%，且最低可辨認之癌細胞於正常細胞下之濃度達1/10,000。
此二維檢測平台的開發未來將與本計畫中前段的三維介電泳分離系統結合，除此之外也可應用在其他需要將細胞作平面觀察的實驗上，用途十分廣泛。

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