中文摘要

本研究中，我們以人類軟骨細胞來探討LMW-CS或IL-1β對基因的影響。我們以及時偵測聚合酵素鏈鎖反應觀察到，IL-1β可誘導MMP1、ADAMTS5、COL2A1、AGC1、COX1、COX2基因的增加，抑制 COL1A2基因表現，但是在顯微鏡觀察下，細胞呈肥大或紡綞狀，此時細胞最終應該是降低第二類型膠原蛋白和蛋白聚糖的分泌；若合併LMW-CS處理細胞，雖然顯微鏡下觀察到LMW-CS並無法制止IL-1β所造的細胞肥大狀況，但是我們以及時偵測聚合酵素鏈鎖反應觀察到LMW-CSA可以抑制IL-1β所誘導的MMP1、ADAMTS5基因的表現，而LMW-CSC並無此現象。雖然以LMW-CSA處理細胞亦可降低MMP1基因表現，然而我們以西方點墨法分析後發現，LMW-CSA並非透過抑制p-ERK1/2而達到MMP1基因表現量的降低；我們也將IL-1β合併Intact-CSA處理細胞，在聚合酵素鏈鎖反應卻無法觀察到Intact-CSA抑制IL-1β誘導的MMP1、ADAMTS5。由以上實驗了解LMW-CSA能抑制背景值和IL-β誘導的MMP1基因表現，並非透過抑制P-ERK1/2的量所達到，而Intact-CSA不能抑制背景值和IL-β誘導的MMP1基因表現。

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Abstract

In this study, we investigate the role of LMW-CSA, LMW-CSC or IL-1□-mediated gene expression in cultured human articular chondrocytes. We analyzed IL-1□-mediated gene expression by quantitative real-time polymerase chain reaction (Q-PCR), MMP1, ADAMTS5, COL2A1, AGC1, COX1, and COX2 were induced, but COL1A2 were down-regulated by IL-1□ treatment. We also observed a morphological change after treating with IL-1□. However, this morphological change of IL-1□□exposed cannot be recovered in the present of LMW-CSA or LMW-CSC. As analyzed by Q-PCR the IL-1□-induced MMP1 and ADAMTS5 gene levels can be administration of LMW-CSA, but not LMW-CSC. Althout LMW-CSA reduce IL-1□-mediated or basal level of MMP1 gene expression by Q-PCR, these inhibitory effect are not acted through p-ERK1/2 pathway. We also investigate whether Intact-CSA is able to reduce IL-1□-mediated MMP1 and ADAMTS5 gene expressions. The result showed that Intact-CSA could not inhibit IL-1□-induced MMP1 and ADAMTS5 gene level.

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