本研究主要是藉由氣/液界面混合單分子層界面性質之研究幫助了解分子間的交互作用。在不同溫度下，分別進行Distearoyl- phosphatidylcholine (DSPC)/cholesterol，DSPC/Distearoylphosphatidyl- ethanolamine-poly(ethylene glycol)2000(DSPE-PEG2000)，DSPC/paclitaxel 混合單分子層，DSPC/cholesterol/DSPE-PEG2000混合單分子層，以及DSPC/cholesterol/DSPE-PEG2000/paclitaxel混合單分子層表面壓-每分子佔據面積(□□A)等溫線之量測。□□A等溫線顯示這四種成分在24.5℃下氣/液界面是互相混合的。藉由所獲得之數據可進行這些混合系統氣/液界面熱力學特徵的研究。溫度與組成兩者對於混合單分子層的等溫線形狀，可壓縮性，面積鬆弛行為以及穩定性均會有顯著的影響。最穩定的配方可結由混合過剩自由能的估算來決定。除此之外，這些混合系統微脂粒膜的穩定性則是藉由微脂粒包覆5,6-carboxyfluorescein(CF)的釋放實驗來判斷。最後，本研究對於這些混合單分子層的發現與他們在微脂粒中的行為也進行了進一步的探討。

This study is primarily concerned with the molecular interaction and the interfacial properties of mixed monolayers at the air/water interface at different temperatures. Measurement of surface pressure □□area per molecule (□□A) isotherms for DSPC/cholesterol, DSPC/DSPE-PEG2000, DSPC/paclitaxel, mixed monolayers, DSPC/cholesterol/DSPE-PEG2000 mixed monolayers, and DSPC/cholesterol/DSPE-PEG2000/paclitaxel mixed monolayers, respectively were conducted at different temperatures. Thermodynamic characteristics of these mixed systems at the air/water interface were studied from data obtained. The □□A isotherms showed these four components were miscible at 24.5℃. Temperature and compositions both had profound influence on the shape of isotherms, the compressibility, the stability, and the relaxation behavior of mixed monolayers. The most stable formulation can be determined by the estimation of the excess free energy of mixing. Additionally, the corresponding liposomal membrane stability of these mixed system was determined by release of encapsulated 5,6-carboxyfluorescein. Finally, the relevance of the observations for these mixed monolayers to the behavior in a liposomal domain was discussed.

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