在創傷弧菌的小染色體上有一套編號為 vva0326-9 的基因群，能產生與霍亂弧菌 VieSAB 三分子調控系統序列相似的產物。其中 vva0328 會表現含有 EAL 功能區域的蛋白質，而 vva0326 則表達含GGDEF 功能區域的蛋白質。由之前的研究發現具 GGDEF 功能區域的蛋白及 EAL 功能區域的蛋白分別具有雙鳥苷酸環化酶和磷酸雙酯分解酶的活性，可調控環狀雙鳥苷酸含量。本實驗室過去研究曾發現 vva0328 缺損的突變株具有較低的細胞毒殺能力及較慢的泳動力。為進一步了解此基因的功能，本研究先構築大量表現 VVA0328 的重組蛋白，並測試其酵素活性，結果並無法偵測到 VVA0328 具有磷酸雙酯分解酶活性。本研究中也構築了 vva0328 突變株的單套互補株，並發現在各種生物表現型試驗中，能恢復突變株原本較低的細胞毒殺能力及泳動性。而利用反轉錄 PCR 的方法也發現在 vva0328 缺損株中，創傷弧菌的主要致病毒素 rtxA 與蛋白酶基因 vvp 都會降低表現活性。但是在溶血性、捕鐵載體產生量以及蛋白酶活性試驗中，vva0328 突變株與野生株並沒有顯著的差異。根據本研究的結果，可以推測 VVA0328 很可能藉由調控 rtxA 及vvp 的基因表現來影響創傷弧菌的致病力。

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