心血管疾病為全球死因之首，而其中急性心肌梗塞 (Acute myocardial infarction , AMI ) 最為難以預測且致命。近年來大眾對於預防醫學的觀念逐漸興盛，因此疾病突發前的預防檢測就相當重要，本研究旨在製作出用於靈敏檢測急性心肌梗塞中心血管生物標誌物「心肌肌鈣蛋白I」(Cardiac Troponin I , cTnI) 的生醫感測器。
近年來，隨著二維材料的發展，越來越多新興的二維材料受到半導體產業的關注。二維材料具有高導電性、高強度和良好的電化學性質等優點，不僅在半導體產業受到關注，還在生醫感測領域有所應用。在眾多二維材料中，二硫化鉬(MoS₂)是最受關注的材料之一，因其穩定性、優良的電學和光學特性以及高載子遷移率，適合用於生醫感測器元件的開發，可為生醫感測領域帶來良好的發展。
本研究所開發的生醫感測器元件，是先在二氧化矽基板上生長連續且大面積的二維MoS₂薄膜並對其進行物理、光學和電學特性等方面的分析研究。隨後，對二維MoS₂薄膜進行元件的圖形化設計。為了提升檢測靈敏度，我們選擇在二維MoS₂元件上加入表面電漿子奈米材料 (Yolk-shell AuNRs@Au/Ag)。表面電漿子奈米材料能有效提升整體比表面積，有助於後續抗體的修飾，同時也能增強二維MoS₂生醫感測器元件的導電性。，進一步降低其檢測極限。
本研究的目標在於開發一種非侵入式的生醫感測晶片，用於檢測急性心肌梗塞中代表性的生物標誌物「心肌肌鈣蛋白I」(Cardiac Troponin I , cTnI)，利用場效電晶體元件的優良電學特性變化進行檢測。研究結果表明，在檢測cTnI方面取得了良好的靈敏度和特異性，並實現了最低檢測極限10 pg/mL。我們希望這項開發能夠在預防突發性心臟疾病方面發揮作用，並為生醫感測領域帶來更多的應用價值。

Cardiovascular diseases are the leading cause of mortality worldwide, with Acute myocardial infarction (AMI) being particularly difficult to predict and deadly. In recent years, there has been a growing interest in preventive medicine, underscoring the importance of pre-symptomatic detection of diseases. This study aims to develop a non-invasive biomedical sensor for the sensitive detection of the cardiovascular biomarker "Cardiac Troponin I" (cTnI) associated with AMI.
With the advancement of two-dimensional materials in recent years, there has been increasing attention in both the semiconductor industry and the biomedical sensing field. Among numerous two-dimensional materials, molybdenum disulfide (MoS₂) stands out due to its stability, excellent electrical and optical properties, and high carrier mobility, making it suitable for the development of biomedical sensor devices and contributing to the advancement of biomedical sensing technology.
The developed biomedical sensor in this study involves the growth of continuous and large-area two-dimensional MoS₂ thin films on silicon dioxide substrates, followed by a comprehensive analysis of their physical, optical, and electrical properties. Subsequently, the design of the MoS₂ thin film devices was carried out. To enhance detection sensitivity, surface plasmon nanomaterials ( Yolk-shell AuNRs@Au/Ag ) were incorporated into the MoS₂ devices. Plasmonic nanomaterials can effectively enhance the overall specific surface area, facilitating subsequent antibody functionalization while also improving the conductivity of two-dimensional MoS₂ biosensor devices, further lowering their detection limit.
The objective of this study is to develop a non-invasive biomedical sensing chip for the detection of the representative biomarker cTnI in AMI, utilizing the excellent electrical properties variation of field-effect transistor devices for detection. The results demonstrate good sensitivity and specificity in detecting cTnI, achieving a detection limit 10 pg/mL. It is hoped that this development will contribute to the prevention of sudden cardiovascular diseases and further enrich the applications of biomedical sensing technology.

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