以即時可調變影像控制技術與光電效應現象為基礎，發展出影像介電泳技術，此技術有別於以往介電泳發展受限於既定導電極板外觀，造成流體晶片功能性不彰。影像介電泳利用即時影像的設計、變化、移動來扮演多功能性之角色，在本文中，詳盡探討其基本操作特性包括速度、作用力、影像介電泳有效半徑與操縱粒徑尺寸極限等。利用影像介電泳推動粒子速度最快可達80 □m/s，在10 Vpp、1 kHz下，所受介電泳力為19 fN，而影像畫素影響的有效半徑約為200 □m。利用此技術再整合操作環境，發展出結合動、靜態流場之影像介電泳晶片(image dielectrophoresis chip)，此晶片可用來操控細胞(酵母菌、藍綠藻與大腸桿菌)及粒子(聚苯乙烯)等標的物。影像介電泳晶片藉由影像設計與配合流體環境，可擁有強大之功能性，如細胞分離且同步導引、傳輸、聚集、群體與單體的操控、虛擬流道製作等。由於是非接觸式動態操作，標的物的損傷可降到最小，使得此晶片與技術適合運用於生醫之檢測、診斷與分析上，並有潛力發展成微全分析系統(μTAS)。

Image dielectrophoresis (iDEP) has been developed based on real-time reconfigurable image controlled photoconductivity. Compared with the electrode-based dielectrophoresis, the functionalities will not be limited by the fixed geometry of electrodes. In this thesis, the fundamental characteristics of iDEP including velocity, force, effective radius of iDEP and size limitation are discussed in detail. In our experiment, when a particle was operated by iDEP, the maximum velocity was 80 □m/s and the equivalent DEP force was 19 pN. The effective radius of iDEP was about 200 □m. We have developed an integrated iDEP chips for simultaneous operation in both static and dynamic flow fields. This chip can be used to manipulate cells and particles. Based on image pre-designs and environment settings, the integrated iDEP chip owns powerful functionalities including cell separation, transportation, aggregation, single cell or cell flock manipulation, virtual channel guidance, etc. By using iDEP for the manipulation of cells or particles, the damage to the specimen caused by the direct contact will be reduced. These characteristics make this technology as a candidate of medical diagnoses or chemical analysis and make it possible to develop a micro-total-analysis-system (μTAS).

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