中文摘要
抗體是生物體內眾多防禦物質的一種，由血液中的B細胞所產生，當外物侵入生物體時，生物體內之細胞會對此外來物產生反應，經過細胞間的命令傳達後，某些血液細胞會開始進行分化作用，產生對外來物具攻擊性的細胞。每一個細胞只會生產一種抗體，然而這種細胞本身不具有分裂與增殖的能力，因此當此種細胞生產抗體達一段時間後，就會死亡、消失。
而單株抗體研製的發展由70年代開始，這二十年來已經廣泛應用於基礎科學研究和生醫檢驗上，除此之外，臨床上直接使用抗體來治療癌症，改善自體免疫疾病等也有相當的進展和成效。而製備單株抗體的方法最主要仍是沿用自1975年Kohler與Milstein發展出製作單株抗體技術，在小鼠體內打入抗原誘發抗體反應，分離出小鼠B細胞和骨髓瘤細胞來進行融合，篩選出可以製造特定單一抗體之融合瘤細胞株，之後方能進行培養大量的單株抗體，這種融合瘤細胞不但具有癌細胞不斷分裂的能力，又有免疫細胞分泌抗體的能力，是目前生醫應用最廣的技術。在單株抗體應用於癌症治療方法的應用，主要是將藥物連結在單株抗體上，即可利用單株抗體的專一性而將藥物帶至目的地。例如:將可以殺死細胞的藥物與對癌細胞有特異性之單株抗體結合一起，再將此結合物打入癌症病患體內，此時，單株抗體就會將藥物帶到癌細胞處，再發揮藥效將癌細胞殺死，並不會影響到正常細胞，有別於放射線和化療等癌症治療方法。
但是在過去使用聚二乙醇化學融合或隨機性電融合製造出的融合瘤細胞，其融合率及品質都十分不穩定，而本論文擬針對單株抗體的製作技術改良，利用半導體製程的技術，在微米尺度下的微流體晶片，設計並完成一具有大規模細胞配對及融合能力之細胞電融合晶片，精準的將欲融合的細胞進行細胞配對與接觸，再搭配輔助電極以電融合的方式，使細胞膜分子短暫產生排列的改變再恢復而相融完成細胞融合。

Antibodies are large Y-shaped proteins produced by B-cells. Antibodies are recruited by the immune system to identify and neutralize foreign objects like bacteria and viruses. The stimulated B cell performs repeated cell divisions, enlargement and differentiation to form a clone of antibody secreting plasma cells. Through specific antigen recognition, clonal expansion and B cell differentiation, effective number of plasma cells are formed and secrets the same required antibody. That antibody then binds to the bacteria making them easy to be ingested by white cells. Each cell can only produce an antibody. However, such cell division and proliferation do not have the capacity. So after a period of time when such cells produce antibodies, they will die and disappear.
The monoclonal antibodies have been widely used for the last twenty years for scientific research and biomedical testing. The use of monoclonal antibodies for cancer treatment and autoimmune diseases shows considerable progress and effectiveness. The main preparation technology of monoclonal antibodies has been developed by Kohler and Milstein in 1975. Injection of the antigen into the mice induces the reaction of antibody. Separating the B cell from the mice and fusing them with the Myeloma cells could filter out the Hybridoma cell line which can generate the specific single antibody subsequent product a large number of monoclonal antibodies. The hybridoma cells, which not only have the ability of cancer cells to keep dividing but also have the ability of the immune cells to secrete antibodies, are the most widely used technology in biomedical presently. Applications of monoclonal antibodies used in cancer treatments and the drug link in monoclonal antibodies; can take the specificity of the monoclonal antibody drug to the destination. For example, the drugs which can kill cells and cancer-specific monoclonal antibody could be combines; and then injected into a cancer patient's body. At this point, the drug will be taken to the cancer cells by the monoclonal antibodies. Finally, playing the pharmacodynamics will kill cancer cells and not affect normal cells, unlike cancer treatments via radiation and chemotherapy, for example.
In the past, either chemical fusion or electric fusion was used to produce hybridoma cells. The fusion efficiency and quality are unstable. This research is intended for developing the microchip technology for high throughput production of monoclonal antibodies. Semiconductor process technology was used to fabricate the micro device on which precise cell pairing and cell fusion can be done.

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