生活於二十一世紀的國人，為了迎頭趕全球性科技時代快速變遷的腳步，往往忽略了自身的心靈舒緩以及良好的生活品質。無論是肉體上各種疾病或是心理上所造成的無形壓力，皆間接的影響孕育下一代新生命的機率。為了解決不孕的症狀，治療相關之生殖醫學技術，一直以來都是被受高度關注的議題之一。目前生殖醫學的發展已能以體外受精的方式，培育出受精卵，並植回母體，也就是所謂的試管嬰兒。可是，事實上除了手術價格昂貴外，成功機率也只有約36.5%。

近年，隨著微機電的發展，可於微流體晶片上共養子宮內膜細胞及受精卵。藉由存於晶片中的多樣系統，模仿人體的運作。以動態連續流體取替血管，給予細胞新鮮養分，建立一個仿生人體子宮之環境平台。即便人體子宮實際的情況與平台仍存在一定的差異性，但仍然可提供一個基礎的模擬平台，便於相關人員對於共養條件下之受精卵進行更深一步的探討及研究。

本研究設計為一模擬人體子宮的平台。利用微流道晶片的技術，製作出同時兼具被動式混合器效果及產生六種不同濃度梯度的上流道，並藉由上、下流道中間直徑17μm之圓孔多孔性PDMS薄膜，形成一個仿微血管的擴散系統，使得下流道的培養液能持續的擴散至生長於底層的子宮內膜細胞。目標為利用3-D微流道，整合濃度梯度及擴散系統，進而共養子宮內膜細胞及受精卵，模擬人類子宮內的實際情形，提高受精卵分裂品質，增加植回母體之妊娠率和分娩率。

People living in the 21st century need to catch up the rapid change pace of global technology and often ignore the soothing of their own soul and the quality of life. The effects coming from either physical disease or psychologically invisible pressure might result in indirect influence to the next generation of new life. In order to solve the infertility symptoms, the treatment of reproductive medical technology has always been a subject to one of the concerned topics. The reproductive medicine IVF is now ready to nurture the embryo in vitro and be implanted in the mother to have test-tube baby. In fact, not only the fees are expensive for the treatment but also the probability of success is only about 36.5%.

In recent years, with the development of Micro-Electro-Mechanical Systems (MEMS) the technology allows to co-culture the stromal cells and the embryos on microfluidic chips. The use of dynamic continuous fluid instead of blood vessels could mimic the function of giving fresh nutrients to establish a bionic uterine environment platform. Even though there are still some differences on the actual situation of uterus and platform, it could provide a biomimicking platform to facilitate the development of embryos which are cultured with stromal cells.

This study was to design a platform simulating the human uterus. By MEMS technology, the gradient generator was designed and implemented with passive micromixers to produce six different medium concentrations on chip in this study. The porous PDMS membrane with 17μm holes between top and bottom channel was utilized to build a diffusion system to mimic vascular. The goal of this research is to use the 3-D microfluidic chip with the function of gradient and diffusion system to co-culture the embryos and stromal cells. This study is to mimic the actual situation of human uterus to improve the quality of embryo splitting to increase the successful rate of pregnancy.

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