農作物的生長及收穫量取決於水份和養分的供給及溫度的變化。在外界環境壓力下，植物活化特定基因來抵抗逆境。在缺水和低溫的狀況下會誘導第二型的LEA蛋白(late embryogenesis abundant protein)，又名脫水蛋白(dehydrin)，會累積在植物體內。本實驗室已將綠豆脫水蛋白基因分離出來並發現當膨壓在0.1 MPa時基因的表現量會遽增。本論文的目的在於分離和分析綠豆脫水蛋白基因的啟動子區域及分析不同啟動子區域的活性，其方法是不同區域的啟動子片段構築於報導基因β-glucuronidase (GUS)之前，並作暫時性的表達將DNA導入大豆原生質體，並利用GUS表現量分析啟動子的強度，在未經任何逆境處理下，綠豆脫水蛋白啟動子的強度大約為花椰菜鑲嵌病毒35S啟動子的十分之一。另一方面，我們也將構築好的載體轉殖至阿拉伯芥中，同樣利用GUS表現量分析啟動子的活性，發現在受粉後第六天活化並持續至種子成熟。另外，分析轉殖植株不同區域啟動子發現，−998 to −843這段區域對於抑制綠豆脫水蛋白基因的表現扮演重要角色。

Plant and crop production are influenced by water accessibility, nutrition supplement and temperature fluctuation. To cope with environmental challenges, a huge set of genes is transcriptionally activated. Many proteins accumulated under dehydration and/or low-temperature conditions, there are also at the same time such as the group 2 LEA protein dehydrin. The mungbean (Vigna radiata) dehydrin VrDHN was isolated previously by our laboratory members. The VrDHN mRNA level was found to increase remarkably when the turgor potential of plant reaches 0.10 MPa. In this study the promoter of the VrDHN gene was isolated and characterized. To characterized the promoter activity, a series of constructs containing different promoter regions fused to the β-glucuronidase (GUS) reporter gene were transiently and stably transformed then subject to histochemical and fluorometric assays. Through transient assay and histochemical and fluorometric assays. The results from transient assay showed that the VrDHN promoter processes about 1/10 activity of the CaMV35S promoted. Analysis of the transgenic Arabidpsis plants indicated the VrDHN promoter is activated at 6 DAP then the activity in seeds after maturation. The VrDHN promoter activity diminished soon after germination. Deletion analysis showed that the −998 to −843 region is important to repress the VrDHN expression.

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