藥物傳遞在癌症治療方面一直是很重要的研究領域之一，開發一個高專一性及低副作用的藥物傳遞系統是必須的。單核球可以滲透至腫瘤組織並累積在缺氧區域中，同時單核球可以穿越血腦屏障，成為一個有潛力的細胞藥物載體。為了探討這個可行性，本研究利用巨噬細胞細胞株 (RAW) 以及C57BL/6J小鼠骨髓細胞在體外分化成單核球 (BMDM) ，給予包覆臨床抗癌用藥Doxorubicin高分子藥物載體poly(AAc-co-DSA) 以及超音波影像對比劑Perfluoropentane droplet，並測試在不同的環境下移動的能力，最後藉由照過放射線的腫瘤條件培養液進行單核球體外訓練。實驗結果顯示，骨髓分化單核球擁有良好的藥物載體攜帶能力，單核球吞噬超音波影像對比藥物後並不會導致載體瓦解及功能性的喪失，同時骨髓分化的單核球有極佳移動至腫瘤微環境的能力。經由照過放射線的腫瘤條件培養液體外訓練後的單核球，其移動至腫瘤微環境的能力顯著提高，同時可以彌補單核球攜帶藥物後移動能力的不足，並且細胞在移動之後並會導致藥物的流失。在活體實驗中確實可以觀察到綠色螢光表現蛋白單核球在經由偉靜脈注射後確實會滲透至腫瘤組織中。我們成功的利用體外訓練的方式，建立了一個以細胞作為藥物載體的系統，並且有極大的潛力運用在活體系統之中。

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