生命系統具有高度的複雜性，各領域的研究學者常採用不同的研究技術，探索生命架構中尚未發掘的運作奧秘。在生物物理相關研究範疇中，藉由光電技術的輔助，重要的理論參數可由實驗資料獲得佐證；而舉凡巨觀及微觀尺度，溫度參數皆在其中扮演重要角色。在雷射光鉗系統中，因使用高度聚焦的雷射光束進行單分子層級的操控與觀測，溫度效應亦為此領域廣泛研究的主題。
半導體相關產業的製程技術在本世紀發展迅速，電子元件的線寬由毫米、微米，減小至奈米尺度。實驗裝置的微小化可由製程技術設計達成，讓使用的樣本體積減少至微升等級；結合製程技術，雷射光鉗的單分子研究更相得益彰。
本篇研究使用半導體微影技術，於微流道腔體中製作微米尺度的圖形化金屬薄膜，整合應用至雷射光鉗系統。經由光路設計，高度聚焦的雷射光束打入微流道腔體中之特定位置；由於圖形化金屬薄膜的吸收及傳導特性，雷射聚焦處將產生升溫現象，並形成不同的溫度梯度分佈於腔體中。藉由探討生物分子於此溫度場中的構形變化，可反應出腔體中溫度及對流行為的分佈，進而於特定加熱點整合雷射光鉗系統，達到生物單分子熱運動行為觀測之研究目的。

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