過去的蛇毒金屬蛋白酵素大部分都發現在出血性的蛇毒，在神經性的毒蛇找到的只有幾種，又多以眼鏡蛇為主。而眼鏡蛇中金屬蛋白酵素作用主要與免疫系統相關和凝血系統相關，其中Cobrin被指出和引發發炎的補體系統相關；Kaouthiagin和凝血系統中的溫韋伯氏因子(vWF)有作用；Mocarhagin則會切白血球表面的PSGL-1和血小板表面的GPIbα，PSGL-1為各種白血球由血液進入組織重要的配體。而GPIbα則有和vWF結合的能力。
本實驗針對台灣眼鏡蛇毒進行篩選，找到一種金屬酵素，並利用胺基的序列比對，確定了在台灣眼鏡蛇中蛇毒金屬蛋白的存在。其包含的金屬離子為三個鈣離子及一個鋅離子。這個新的金屬酵素對於具有硫化的PSGL-1，有較強的分解能力，由此推測此蛋白可能作用在發炎反應中白血球進到發炎組織滾動黏附的過程。

Abstract

Snake venom metalloprotease (SVMP) found in the past is mostly in the hemorrhagic snake venom. Several recent reports indicate that SVMP may also be present in snake venom known to mainly perturb nerve systems. Most of them are found in cobra venom. The function of SVMP in cobra is mainly concerned with immune system and Hemostatic system. Belonging to one of two system, Cobrin is regarded to be related to complement system which triggers inflammation, Kaouthiagin reacts with von Willebrand factor, vWF, in Hemostatic system, and Mocarhagin is able to digest PSGL-1 of neutrophils and GPIbα of platelet. PSGL-1 is an important ligand which affect each type of neutrophils to enter endothelial surface. Furthermore, GPIbα has the ability to bind with vWF.
In this thesis we screen the possible existence of SVMP in Taiwan Cobra by monitoring fibrinogenolytic activity. A new SVMP with Mocarhagin-like activity, but with Cobrin-like sequence, is identified. This protein contains Ca2+ and Zn2+ with molar ratio of about 3 to 1. Taiwan SVMP exhibits stronger protease activity against sulfated PSGL-1 than non-sulfated one. It is suggested that SVMP from Taiwan Cobra functions by perturbing the rolling and adhesion of neutrophils at the site of inflammation.

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