生物質燃燒所排放之汙染為無論是氣相或固相，對整個環境的污染是全球性的。經由生物質燃燒所產生的氣膠大小多落在直徑2.5微米以下(PM2.5)，並且對於全球環境貢獻相當大比例的有機顆粒。這些顆粒物在大氣當中生命週期長，可經由遠程傳輸影響周邊相關空氣品質。尤其在美國西部以及西北部，每年所發生森林大火的數量和面積都是相當可觀的數字。此研究中，採集樣品週期為2001年到2004年總共3.5年，採樣地點為美國科羅拉多州柯林斯堡。本研究藉由分析水溶性有機碳，有機碳，無機碳，無機離子以及脫水糖來了解當地空氣的污染來源。結果也顯示生物質燃燒示蹤物(左旋葡萄醣聚醣(levoglucosan)、甘露醣聚醣(mannosan)、半乳醣聚醣(galactosan))以及二次性有機氣膠的示蹤物(2-甲基丁四醇(2-methyltetrols))有明顯的四季變化，其中生物質燃燒示蹤物比值(LG/MN)可以當作推測燃燒來源的依據，結果顯示軟木為主要冬季生物質燃燒來源。左旋葡萄醣聚醣和有機碳的比值(LG-C/OC)為一常見用來定量估算生物質燃燒貢獻的方式，冬季平均生物質燃燒占總有機碳為15.3%，夏季僅針對有森林大火期間作計算。水溶性有機碳來源主要有兩個：二次有機氣膠以及生物質燃燒排放(包含一次以及二次性氣膠)，其濃度夏季可達冬季十倍之多。有機碳和無機碳為主要細顆粒(PM2.5)組成成分。其中生物質燃燒所排放的有機碳無機碳是相當多的。有機碳無機碳的比值也是經常用來討論污染來源的方式，生物質燃燒所產生的有機碳無機碳比值會大於石化燃料所產生的比值。2003樣品也使用新的TD-2D-GC-IRMS/MSD方法做同位素碳13的分析。
δ13C亦可幫助判定燃燒來源，其分析結果與生物質燃燒示蹤物比值結果相呼應。但是此方法在研究的困難點在於基質效應嚴重，其對於定性分析結果不僅影響分析物的滯留時間，也讓分析物不易被分離。水溶劑取代甲醇為降低基質效應，並使用冷凍乾燥法(freeze drying method)除去水份後進樣。結果顯示沒有同位素分餾現象，且確實有效降低基質效應。

Biomass burning activities constitute a major source of both gaseous and particulate pollutants on global scale. Aerosol particles released from biomass burning processes typically have sizes of less than 2.5 µm aerodynamic diameter (PM2.5), and they constitute a particularly large source of organic particles on global scale. Those particles can be transported over long distances and impact the air quality even in remote areas. Especially the Western and Northwestern US experience numerous and large-scale wildfires in each year. In this study, samples were collected from 2001 to 2004, totally 3 and half years in Fort Collins, Colorado, US. Water-soluble organic carbon (WSOC), organic carbons (OC), elemental carbons (EC), inorganic ions, anhydrosugars were analyzed in this study. Biomass burning tracers (levoglucosan, mannosan and galactosan) and secondary organic aerosol tracers (methylerythritol and methylthreitol) clearly showed seasonal pattern. The biomass burning tracer ratio (LG/MN) can be a fingerprint for the burning sources. The result showed the burning sources in winter were softwood. Levoglucosan to organic carbon ratios (LG-C/OC) were used to quantitatively estimate the contributions from biomass burning to ambient PM. 15.3% of the OC came from biomass burning in winter average. WSOC has been found to have two main sources: SOA formation and biomass burning emissions (both primary and secondary). The concentration in summer is 10 times higher than winter, which was discussed in the study. OC and EC aerosol components typically account for a significant proportion of fine particulate matter (PM2.5) in the atmosphere. Biomass burning can generate large amounts of OC and EC, and OC/EC can also provide fingerprints for the type of emission source. For example, biomass burning is usually higher than those from fossil fuel combustion source. One part of the samples were analyzed by the novel TD-2D-GC-IRMS/MSD as well, in order to obtain compound-specific carbon isotope composition. δ13C values can be a fingerprint for the burning sources, which showed good agreement between the biomass burning tracer ratios. Serious matrixs effect make it harder to do quantitative analysis, which not only influences the retention time but also makes the tracers not easy to be identified. In order to lower the matrixs effect, water was used as solvent for extraction, followed by freeze drying to dehydrate the samples. No isotopic fractionation occurred with this new method, and the resolution was significnatly impoved.

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