本研究主要探討設計電阻式微型加熱線圈(Micro-Heater)於介電濕潤(Electrowetting on dielectric, EWOD)晶片上蓋之方法做為操控液珠的平台，用以檢測生物單一核苷酸多型性(Single-nucleotide Polymorphism, SNP)是否發生於特定的基因片段。此設計提供EWOD晶片在操控液珠時達到預設之溫度環境，使特定生化反應足以發生，搭配EWOD系統能夠有效率的產生、移動、混合與分離DNA、磁球與緩衝液等檢體液滴之功能，目標是在EWOD晶片上完整呈現SNP檢測實驗，達到Lab on a chip之目標。
電阻式微型加熱線圈之設計是利用透明玻璃為基板，在其上沉積金屬導線並沉積絕緣層後可製作出加熱線圈晶片，再經由紅外線熱像儀(IR Scope)量測，可得知不同施加功率對於溫度變化之關係。並將其整合於EWOD晶片之上蓋結構，並透過特殊的線路設計使得微加熱線圈能夠符合EWOD晶片上所需的加熱位置，經由對準封裝後完成微型加熱器晶片與EWOD晶片之整合。本研究設計之微加熱線圈將在SNP檢測之核心步驟提供去氧核醣核酸接合酶(DNA Ligase)反應的適當溫度環境，最終將在晶片上完成SNP檢測實驗。

This study bases on the technique, “Electrowetting on Dielectric (EWOD),” and we design micro-heaters on the cover of a EWOD chip as proper temperature sources for biomedical detection. Droplets in a EWOD chip are actuated by addressable electrodes. Multiple bio-reagents such as DNA, nano-magnetic beads and buffer droplets have been manipulated by the EWOD system already. Due to the nano-magnetic beads property that can be collected and purified, it can use to detect a single-nucleotide polymorphism (SNP) in specific gene sequence. Above these, the final goal of this research is efficiently using temperature-controllable EWOD digital microfluidics system for SNP detection in specific gene sequence.
The micro-heaters are fabricated on glass substrate by Pt deposition. After the heater circuit design, they are constructed with bottom chip to complete the packaging of temperature-controllable EWOD system. In this study, micro-heaters in a EWOD system can provide proper temperature for DNA ligase in SNP detection experiments. In the end, we successfully use temperature-controllable EWOD digital microfluidics system to detect SNP code in specific gene sequence, and this research will contribute to biomedical diagnosis.

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