我們介紹了一種低花費且可表達細菌對於抗生素的進化途徑的培養裝置，這個裝置可連續的觀察細菌的成長過程，為了驗證這個系統，我們逐步的測量Escherichia coli MG 1655對於trimethoprim的抗藥性，並置入多重微流體裝置中並測量大腸桿菌的型態變化和抗藥性，該方法可以延伸到實驗室的抗生素耐藥性研究，並擴展到其他的適應性進化和細菌培養實驗。

We describe a low cost, configurable bacterial culture device for characterizing the evolutionary pathway of antibiotic resistance. This device continuously monitors the evolving bacterial growth. To validate the platform, we measure the stepwise acquisition of trimethoprim resistance in Escherichia coli MG 1655, and integrate the platform into a multiplexed microfluidic platform for investigating cell morphology and antibiotic susceptibility. The approach can be up-scaled to laboratory studies of antibiotic drug resistance, and is extendible to other adaptive evolution and bacterial culture experiments.

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