摘要

AMPA receptors在大腦中調控大部分快速的興奮性神經傳導物質，缺乏GluR2 的 AMPA receptors 會表現Ca2+ - permeable，這些受器會分布在大腦各個不同部位，有一些會表現在抑制性的 GABAergic interneurons 上。而在大腦皮質發育的早期，隨著發育的時期不同，也需要不同的AMPA receptors的活性。有些研究發現，在老鼠大腦發育早期大約出生後12到16天，AMPA receptors表現型為Ca2+ - permeable，但到了晚期 ( 出生16天之後 ) ，就為Ca2+ - impermeable。在這裡我們以胎鼠( E18 )取得大腦皮質神經細胞，進行離體無血清神經細胞培養，在培養第一週 ( 7DIV或8DIV ) 、第二週 ( 14DIV )時，以電生理的方法記錄突觸上麩胺酸受器的表現情形。我們利用high/low calcium交換實驗比較培養第一週 ( 7DIV或8DIV ) 與第二週 ( 14DIV ) 的神經元突觸上AMPA receptors對於Ca2+通透性表現的情形，結果發現，第一週的AMPA receptor不具有讓Ca2+通透的特性，而發育至第二週時，AMPA receptor亦不具有讓Ca2+通透的特性。顯示第一週及第二週時突觸上的AMPA receptor大多含有GluR2 subunit。

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