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研究生： 廖良文
Liao, Liang-Wen
論文名稱： 以場效電晶體感測器定量檢測人類乙型絨毛膜促性腺激素
Electrical Double Layer Gated Field Effect Transistor Biosensors for Quantitative Detection of Beta-Human Chorionic Gonadotropin
指導教授： 王玉麟
Wang, Yu-Lin
口試委員: 林宗宏
Lin, Zong-Hong
陳榮治
Chen, Jung-Chih
學位類別： 碩士
Master
系所名稱： 工學院 - 動力機械工程學系
Department of Power Mechanical Engineering
論文出版年： 2020
畢業學年度： 109
語文別： 英文
論文頁數： 48
中文關鍵詞： 生物感測器 、場效電晶體感測器 、即時檢測 、人類乙型絨毛膜促性腺激素 、懷孕檢測 、子宮外孕
外文關鍵詞： biosensor, EDL-FET based sensor, point of care, ß-hCG, pregnancy test, ectopic pregnancy
相關次數： 點閱：4  下載：0 
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    • 在此研究中，我們團隊開發一種手持式的生物感測器，用於檢測人類乙型絨毛膜促性腺激素，此激素為日前廣泛使用於妊娠試驗的胎盤激素，亦可作為許多疾病的生物標誌物，例如異位妊娠、睾丸癌和唐氏症等等，而此生物傳感器的檢測方法是利用電雙層閘極門控場效電晶體施加偏電壓，測量最終的漏極電流信號，並以數位訊號形式於螢幕顯示結果。另外，亦可以應用螢光測量技術做表面修飾的分析和確認。
    該生物感測器具有良好的偵測範圍及很低的檢測極限，這代表我們的傳感器具有在很早的階段檢測到疾病的潛力。此外，此傳感器可以批量生產，亦無需使用其他標記試劑或樣品預處理之技術，並且能居家隨時檢測追蹤，對於妊娠婦女追蹤協助觀察孕期十分有幫助。

    In this research, a portable biosensor was developed to detect beta-human chorionic gonadotropin (ß-hCG) which is a placental hormone widely used in pregnancy tests and serves as a biomarker for diseases such as ectopic pregnancy and testicular cancer. The sensor that we used is an electrical double layer (EDL) field-effect transistor (FET) biosensor with the extended-gate design. we can apply the bias voltage on the sensor to measure the resulting drain current signal and display the output results. The gold electrode surface is functionally activated with an anti-hCG antibody to capture the ß-hCG protein.
    Fluorescence measurement technique is introduced to analyze and confirm the surface functionalization. The developed biosensor demonstrates a dynamically wide range of molecules as detection targets at very low concentrations, which shows the potential of our sensor to detect ectopic pregnancy in very early stages and track its daily changes easily. It can be produced in batches and does not use additional labels/reagents or pre-processing techniques for the sample. This biosensor can significantly enhance the cost-effectiveness and is comparable with the current commercially available ß-hCG assays.

    摘要 i ABSTRACT ii LIST OF TABLES v LIST OF FIGURES vi Chapter 1 INTRODUCTION 1 1.1 Motivation 1 1.2 Objective 5 Chapter 2 LITERATURE REVIEW 6 2.1 Field Effect Transistor biosensor 6 2.2 Electrical Double Layer 7 2.3 Beta-Human Chorionic Gonadotropin 9 2.4 Pregnancy and Ectopic pregnancy 10 2.5 Testicular cancer 12 2.6 Comparison of detection methods for ß-hCG 13 Chapter 3 Experimental Design 14 3.1 Extended gated sensor array chip fabrication 14 3.2 Surface functionalization 15 3.2.1 Surface cleaning 15 3.2.2 Functionalization method 16 3.3 Measurement method 19 3.4 Fluorescence experimental confirmation 21 Chapter 4 RESULTS AND DISCUSSION 22 4.1 MOSFET characteristics 22 4.2 Surface clean process improvement 24 4.3 Comparison of surface-immobilized concentration 27 4.4 ß-hCG detection using extended gate EDL FET biosensor 30 4.4.1 Extended gate EDL FET biosensor sensing mechanism 30 4.4.2 Detection in 1X PBS solution 34 4.4.3 Detection in 1X PBS with 4% BSA 37 4.4.4 Detection in Artificial Urine 38 4.4.5 Detection after freeze-drying 39 4.5 Sensor detection of non-specific binding and selectivity 42 Chapter 5 SUMMARY AND FUTURE WORK 44 REFERENCE 45

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