結合微機電系統創造新的工具來探討生物問題已經是現今科學界的一項目標，本實驗室也是致力於發展微接觸壓印方法來進行大規模的神經體與神經纖維次細胞級結構分離，如果此系統可以成功建立，將可能是研究方法上的革命進展。然而，在我們所做的實驗當中，事情並不如預期中順利，一再出現的細胞聚集(aggregation)現象使得我們所培養的細胞生長不受控制。為了解決細胞聚集帶來的問題，我們嘗試了多種實驗方法，包括運用多種蛋白壓印 (膠原蛋白、胞外間質)，以交聯劑修飾基質(substrate)表面，經由自組裝單層分子印製微圖案，利用神經膠細胞共同培養來支持神經生長以及將細胞直接培養於具有圖案的聚二甲基矽氧烷(polydimethylsiloxane；PDMS )基材上，除了神經膠細胞共同培養以外，所有的嘗試幾乎都不能改善細胞聚集問題，共同培養的方法又容易導致非神經細胞污染，因此也不適合使用。直到我們採用PDMS模板來作為限制細胞貼附區域工具，細胞聚集現象才得以解決，而經由模板培養，我們也意外發現細胞聚集的出現成因，可能是來自於微接觸壓印實驗中，無法讓細胞貼附區域過多，導致漂浮細胞於培養液中，形成細胞團塊後，才沉降到細胞貼附區。經由我們所設計的實驗證實，這些漂浮細胞的確具有貼附能力，且隨著無法貼附區的面積越大，細胞聚集現象越嚴重。而目前配合模板所做的微接觸壓印方法，已經成功培養出正常生長的神經細胞，經由免疫染色觀察，神經纖維末端的生長錐也可以經由此方法限制在微圖案細線末端。接著，只要再稍微調整微圖案設計及模版製作方式，我們將可以得到大量生長錐，以及嘗試進行前突觸的誘導分化，並進行有系統的蛋白質體分析，以研究生長錐發育時的變化及突觸分化的分子機制。

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