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研究生： 林重佑
Chung-Yao Lin
論文名稱： 血糖感測器與分段採樣檢測微流道之設計與研製
On the Design and Research of the Glucose Sensor and Sequent Sampling Microfluidic system
指導教授： 曾繁根
Fang-Gang Tseng
楊重熙
Chung-Si Yung
口試委員:
學位類別： 碩士
Master
系所名稱： 原子科學院 - 工程與系統科學系
Department of Engineering and System Science
論文出版年： 2005
畢業學年度： 93
語文別： 中文
論文頁數： 75
中文關鍵詞： 血糖計 、酵素電極 、普魯士藍 、分段微流體系統
外文關鍵詞： glucose sensor, enzymatic electrode, Prussian Blue, Sequent sampling, microfluidic
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    • 本篇論文分做兩大部分，其一為完成血糖計之研製與基礎電極特性研究。研究過程中遭遇主要困難在於酵素電極製作與封裝測試問題，經過3代的設計實驗，最後利用普魯士藍/酵素層(酵素+戊二醛+Nafion)，可以將酵素成功低固定於電極之上，並在封閉流道中，以連續抽入的方式檢測血糖，可以得到穩定且線性關係的電流訊號變化。訊號量測極限為5μM，靈敏度為336uA/cm2.mM 。最後探討此血糖計之重複性與再現性。論文另一部分為：分段採血檢測之微流體系統設計。為實現此設計理念，後續完成設計之前置實驗研究，包括：利用雙層SU8負型光阻結構，在開放流道下成功阻擋微通道內流體的前進，接著發展SU8接合技術，並研究加熱製程對電漿處理後的表面親水特性之影響。

    摘要...................................................2 目錄...................................................3 第一章 緒論............................................6 1.1 前言............................................6 1.2 糖尿病..........................................8 1.3 研究目標之1.....................................9 1.4 研究目標之2....................................13 第二章 血糖計之文獻回顧...............................14 2.1 血糖檢測方法...................................14 2.2 血糖計發展歷史回顧.............................17 2.3 植入式血糖計...................................17 2.4 最低限度破壞之侵入式系統.......................18 2.5 非侵入式檢測系統...............................19 2.6 目前現有之血糖計商品.......................... 20 2.7 總結...........................................21 第三章 血糖計之研究歷程與實驗設計....................22 3.1 血糖感測器之測試研發...........................22 3.1.1 第一代設計................................22 3.1.2 第二代設計................................25 3.2 第二代微流道設計...............................27 3.2.1 晶片製備..................................27 3.2.2 第二代設計對照組測試---大尺度電極.........28 3.2.3 第二代設計---晶片測試......................31 3.4 第三代設計......................................33 3.4.1 普魯士藍...................................34 3.4.2 酵素電極置備.............................. 36 3.4.3 封裝製作與測試設備架設.................... 37 3.5 第三代設計測試結果與討論....................... 39 3.5.1 雙氧水訊號量測............................ 40 3.5.2 血糖訊號量測.............................. 41 3.5.3 流量的影響................................ 44 3.5.4 重複性(Repeatability)..................... 46 3.5.5 再現性(Reproducibility)................... 47 3.5.6 靜態測試.................................. 49 3.6 三代設計演進之比較............................. 50 第四章 定時分段抽血檢測裝置之研發.................... 51 4.1 初步設計架構與操作原理......................... 51 4.2 製程與實驗測試................................. 54 4.2.1 緩衝液的填充.............................. 54 4.2.2 SU8接合技術............................... 57 4.2.3 封閉流道之親水處理........................ 59 4.3 實際設計接合測試............................... 62 第五章 結論與未來規劃................................. 66 5.1血糖感測器部份.................................. 66 5.2 分段採樣流道部分............................... 66 5.3 未來規劃....................................... 67 參考文獻.............................................. 69 附錄一 血糖計全血檢測之問題........................... 72 a. 氧化還原反應的干擾 (Redox interference)......... 72 b. 氧氣消耗的問題(Oxygen deficit).................. 72 附錄二 金電極之測試................................... 74 附錄三 以電沉積法固定酵素............................. 78

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