非洲睡蓮是一種會結出異儲型種子之水生植物。它的種子不耐乾燥處理，同時無法在傳統之貯存溫度下穩定保存。因此，本研究嘗試利用液態氮進行非洲睡蓮種子之冷凍保存。種子預先進行玻璃化處理以避免因冷凍保存而產生會導致細胞死亡的冰晶。實驗發現經過12小時與18小時植物玻璃化抗凍配方3 (Plant vitrification solution 3, PVS3) 處理後，冷凍保存之種子出現最高的發芽率 (66.7%)。另外，50% w/v蔗糖 + 50% w/v丙二醇的玻璃化溫度 (-63.6℃) 相對PVS3 (-90.7℃) 呈現最顯著之提升。
台灣水韭是一種半水生植物，它從2004年開始被IUCN列成瀕臨絕種之物種。在室溫下，台灣水韭植株能夠忍受長達1年之陰乾處理；經過4℃保存11個月後，10%的陰乾球莖仍然保持其再生能力，並在復水後重新長出新的葉子與嫩根。不過，經過各種前處理再放入液態氮保存的球莖皆失去其活性。

Nymphaea capensis is an aquatic plant which produces recalcitrant seeds. Its’ seeds were desiccation intolerant and metabolically active. Cryopreservation is suggested because the conventional methods could not provide a stable storage environment for the seeds. Vitrification pretreatment was used to avoid the formation of ice crystals in cryopreservation. This study showed that cryopreserved seeds which progressed 12 h and 18 h of plant vitrification solution 3 (PVS3) treatments brought the highest germination rate (66.7%) among various pretreatment periods. The glass transition temperature (Tg) of 75% w/v sucrose + 25% w/v propylene glycol (PG) solution (-63.6℃) had the largest improvement among the alternative vitrification solutions compared to PVS3 (-90.7℃).
Isoetes taiwanensis is a semiaquatic plant which is listed as a critically endangered species based on the criteria of IUCN. In contrast to the seeds of N. capensis, the plants of I. taiwanensis could tolerate air-dried for ca. one year in room temperature. Air-dried corms were retained 10% survival rate after 11 months storage under 4℃ and able to regrow new leaves and roots after rehydration. However, corms completely lost viability in cryopreservation even if various pretreatments were used.

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