嚴重的組織潰爛與傷口復原困難是眼鏡蛇咬的主要症狀。心臟毒素Cardiotoxin（CTXs）是屬於Ly-6蛋白家族中造成組織壞死的主要成分。眼鏡蛇心臟毒素A3與硫苷脂的作用下會導致細胞膜孔洞化與內吞作用對心肌細胞產生細胞毒性(Wang .et al2006)我們在這裡利用表面電漿共振的方式討論一種無細胞毒性的心臟毒素A5及利用分生技術以大腸桿菌系統表現的重組蛋白與標的物的作用，如肝素以及v整合素；探討其結合強度。結果顯示重組心臟毒素A5與天然心臟毒素A5有著相似的結合強度；除了重組蛋白之外，利用點突變的技術表現突變蛋白對於標的物結合的影響。而我們也同樣利用表面電漿共振的方式探討蛇毒中另一個的成分－磷酸二酯酶（Phosphodiesterase,PDE）與其可能的作用標的物－胰島素受體之間的結合能力。

Severe tissue necrosis with a slow wound healing process is a major symptom of a cobra snakebite. Cardiotoxins（CTXs）are major components of cobra venoms that belong to Ly-6 protein family and are implicated in tissue damage. Cobra Cardiotoxin A3 interacts with sulfatide on cell membrane will cause membrane pore foramtion and cell internalization and to be responsible for cytotoxicity in cardiomyocytes. Here we demonstrate a non cytotoxicity cardiotoxin,CTX A5 and its recombinant protein express by E.coli system interact with target, heparin and vintegrin by Surface plasmon resonance analysis and using point mutation technique to mutate the specific protein to observe the difference in binding intensity. We also show the other component of cobra venom, Phosphodiesterase,PDE interact with its potential target,insulin receptor by SPR.

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李紹禎博士論文　蛇毒心臟毒蛋白與肝素作用之結合專注性與結合模式及其生物意義(2004)