當細胞內部之細胞骨架去貼附細胞外基質時，細胞會產生強烈收縮力去攀爬基材表面，使細胞延展開來並且進行分化的作用，而細胞外基質的結構強度會影響細胞的生理狀況及生物力學特性。當細胞生長時細胞外基質必須有合適的環境來提供良好之生長條件，否則即使細胞與植入表面達到接觸，但是細胞卻無法在基材表面拓展開來，與基材表面產生牢固的貼附效果，在這種細胞骨架結構行為失敗的情況下，細胞可能會邁向凋亡的路徑發展。不同的細胞在同一外基質上展現的細胞力學情況不盡相同，故研究細胞力學可助於利用物理性質探討細胞的動態行為，相當複雜且重要。
本研究在矽基材表面鋪上單層PS球陣列，再透過濕蝕刻製出矽奈米柱陣列，大面積規則排列的垂直柱狀結構可用來偵測細胞爬行的牽引力(Cell traction force)，於奈米柱尖端鍍上金屬薄膜，在暗場環境下可標定柱子位置，藉由柱子頂端的偏移量，可推算回去得知細胞牽引力的大小。過去文獻對於細胞力學的探討多是蒐集數個細胞的分析數據後以統計方式歸納結論，在本研究我們嘗試長時間觀察單一細胞對矽奈米柱的拉伸狀況，並探討細胞各部位的牽引力大小和細胞運動行為的關係。

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