整合型紙式微流體晶片利用磁性複合薄膜元件以應用於細菌檢測

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研究生：	吳佳翰 Wu, Jia-Han
論文名稱：	整合型紙式微流體晶片利用磁性複合薄膜元件以應用於細菌檢測 An Integrated Paper-Based Microfluidic System for Bacterial Detection Utilizing Magnetic-composite-membrane devices
指導教授：	李國賓 Lee, Gwo-Bin
口試委員:	陳致真 Chen, Chih-chen 何宗易 Ho, Tsung-Yi
學位類別：	碩士 Master
系所名稱：	工學院 - 動力機械工程學系 Department of Power Mechanical Engineering
論文出版年：	2017
畢業學年度：	105
語文別：	英文
論文頁數：	63
中文關鍵詞：	細菌檢測、蠕動式幫浦、微流體、複合性薄膜、電磁鐵、微混合器、微閥門、紙式微流體
外文關鍵詞：	bacterial detection, micro-pump, microfluidics, composite membrane, electromagnet, micromixer, micro-valve, paper-based microfluidics
相關次數：	點閱：3 下載：0
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細菌檢測具重要性尤其是在急診室裡避免醫療照護相關感染。在本研究中，我們提出了一種新的致動方法，利用電磁鐵、蠕動式微幫浦、微閥門及微混合器去整合一自動化之微流體晶片適用於細菌檢測。首先，我們設計製造了一個電磁驅動的蠕動式幫浦，幫浦的構成中包含一關鍵的元件-複合性薄膜，它是透過翻模的製程方式製做出來。複合性薄膜是鐵粉和聚二甲基矽氧烷 (PDMS)混合，當在薄膜上施加磁場，可使其產生一變形量。對於不同的參數設定，薄膜變形量的量測已完成。電磁鐵是由800匝的黃銅線微繞於鐵芯製成，其可產生足夠的磁場來致動本裝置。其在施加7.5伏特的電壓時，可以產生121.6 μl/min的傳輸流率。從本研究的結果表示，此電磁式微幫浦是一有前景應用於醫學與生物應用的裝置。此外，我們提出了一個適用於細菌檢測的微流體晶片設計，此晶片整合了磁性致動的微元件如幫浦、閥門、混合器等去執行一個紙式的細菌檢測。整個檢測流程可以在40分鐘內完成，其檢測極限為4.5×102顆鮑氏不動桿菌。我們期望晶片的檢測可得到與手動實驗相匹配的訊號結果。此種新的生物檢測可減少使用儀器大小，並有潛力發展出一可攜式的檢測工具。
關鍵詞：細菌檢測、蠕動式幫浦、微流體、複合性薄膜、電磁鐵、微混合器、微閥門、紙式微流體

Bacterial detection is important especially in the emergency room to prevent healthcare-associated-infections (HAIs). In this study, we presented a new method which used electromagnetic peristaltic micro-pumps, micro-valves and micromixers to form an integrated microfluidic chip for bacterial detection. First, the peristaltic micro-pump was designed and fabricated by using composite membranes which were fabricated by a replication process. The composite membrane was mixed with iron particles and polydimethylsiloxane (PDMS) which could be deflected when a magnetic field was applied. The characterization of membrane deflection with different applied magnetic fields was measured. The electromagnets were made using iron cores surrounded by 800 turns of brass coils which could produce enough magnetic field to actuate the device. The flow rate was measured to be 121.6 µl/min with an applied voltage of 7.5 V. Experimental results showed that the electromagnetic micro-pump could be a promising microdevice for medical and biomedical applications. Furthermore, we presented the design of an integrated microfluidic chip for bacterial detection. The chip integrated with magnetic micro-devices such as micropumps, microvalves and micromixer was used to perform a paper-based bacterial detection. The process could be performed in less than 40 minutes with detection limits of 4.5×102 (Acinetobacter baumannii). The new method of biological assay could minimize the dimensions of the equipment and has the potential for being developed into a portable device.
Keywords: bacterial detection, micro-pump, microfluidics, composite membrane, electromagnet, micromixer, micro-valve, paper-based microfluidics

Table of Contents
Abstract....    I
摘要...........    III
Table of contents    VI
List of figures    IX
Abbreviations    XII
Chapter 1  Introduction……………………………………………….1
1    Infectious bacteria and healthcare-associated infections (HAIs)    1
1.1    Diagnostic methods for infectious bacteria    2
2    MEMS-based microfluidic technology    4
3    Background and literature survey    5
3.1    Micropumps    5
3.2    Magnetic composite materials    9
3.3    Magnetic actuation applications    9
4    Motivation and novelty    10
5    The structure of thesis    12
Chapter 2  Materials and methods......................................................14
1    Chip design and fabrication proces    14
1.1     Composite membrane fabrication    14
1.2     Peristaltic micropump fabrication    15
1.3     Microfluidic chip for bacterial detection fabrication    16
2    Electromagnet manufacture    20
3    Packaging, power supply and control circuit system    21
4    Working principle of peristaltic micropumps    22
5    Experimental setup    23
5.1     Flow rate-backpressure measurement    24
5.2     Experimental process for bacterial detection    25
6     Sample preparation    30
6.1     Bacteria samples    30
6.2     Preparation of the specific aptamer    30
6.3     Preparation of the HRP-conjugated streptavidin    32
6.4     Preparation of the TMB stabilized substrate    32
Chapter 3  Results and discussion    33
1    Characterization of magnetic composite membrane    33
2    Characterization of the membrane deflection    34
2.1    Characterization of composite ratio    35
2.2    Characterization of membrane thickness    37
2.3    Characterization of membrane diameter    38
3    Characterization of the magnetic interference    39
4    Characterization of the flow rate    41
5    Microfluidic chip characterization    43
5.1     Pumping rate of the micropump    44
5.2     Mixing index of the micromixer    45
6    Optimization of the concentration of aptamer    47
6.1     Optimization of the 1st aptamer    47
6.2    Optimization of the 2nd aptamer    49
7    Limit of the detection of acinetobacter baumannii    50
Chapter 4  Conclusions and future works    53
1    Conclusions    53
2    Future works    54
References…...………………………………………………………….55
Publication list………………………………………………………....63


                                

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