本研究為一結合流力聚焦生成液珠以及介電泳力之生醫晶片，並使用聚二甲基矽(PDMS)製作其主要結構，具有製作簡單、防止汙染風險、降低細胞培養成本且具生物相容性等特性。本晶片利用正介電泳力捕捉卵母細胞以及具有活動力的精子，使卵母細胞周圍增加精子濃度。電極為藉由蝕刻氧化铟锡(ITO)所得出的平行結構。接著，利用兩液相流體分層將小鼠卵母細胞包覆於液珠內，以利於體外受精(In Vitro Fertilization, IVF)的細胞培育。包覆受精卵的液珠可透過改變介電泳緩衝液及培養油的流率控制體積大小。
本研究在低精卵比(500及2000)的狀況之下，晶片組較傳統體外組有較高的授精率(約5%)，且能有較高的比率(約20%)發育至囊胚期。在篩選目標液珠與空液珠的部分，本研究可達到100%的篩選率。
另外，本研究也探討傳統體外授精在培養方式上的比較，得出在小體積(8μl)的培養液珠中個別培養受精卵，於低精卵比的條件下具有較好的發育率，相較於大體積液珠培養具有更好的發育率，可多出約10~20%的機率發育至囊胚期。

In this study, we created a simple microfluidic platform that uses with in vitro fertilization (IVF) and avoided unexpected damage to oocytes due to frequent manipulation of the sperms and oocytes in traditional IVF operation. The device in this research could also be used to help reducing medium volumes, cell culture cost, evaporation problem and prevents contamination risk.
To decrease the impact and destruction of the oocyte and the sperm, we adopted a positive dielectrophoretic force to manipulate the oocyte. The mouse oocytes are trapped by the positive dielectrophoretic (p-DEP) force at the ITO-glass electrodes. The ITO-glass electrodes chip was fabricated by the ITO-glass wet etching. And, the polydimethylsiloxane (PDMS) flow-focusing microfluidic device was used for micrometer-size microdroplets generation to make zygote contained. The volume of microdroplets can be controlled by adjusting the flow rates of both oil and DEP buffer. As a result, the fertility rate could be increased about 5% higher with DEP treated than traditional IVF and even more than 20% developed to blastocyst stage with lower sperm-oocyte ratio. The sorting rate could also achieve 100%. Furthermore, the research compared cultivation method in traditional way. The result shows that the development rate for small volume cultivation is about 10% higher than large volume cultivation.

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